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Aktuelle Fachpublikationen

Detecting natural adaptation of the Streptococcus thermophilus CRISPR-Cas systems in research and classroom settings

Nature Protocols 12, 547 (2017). doi:10.1038/nprot.2016.186 Authors: Alexander P Hynes, Marie-Laurence Lemay, Luc Trudel, Hélène Deveau, Michel Frenette, Denise M Tremblay & Sylvain Moineau CRISPR (clustered regularly interspaced short palindromic repeats)-Cas systems hav ... mehr

A modular approach to the design, fabrication, and characterization of muscle-powered biological machines

Nature Protocols 12, 519 (2017). doi:10.1038/nprot.2016.185 Authors: Ritu Raman, Caroline Cvetkovic & Rashid Bashir Biological machines consisting of cells and biomaterials have the potential to dynamically sense, process, respond, and adapt to environmental signals in re ... mehr

Turning single-molecule localization microscopy into a quantitative bioanalytical tool

Nature Protocols 12, 453 (2017). doi:10.1038/nprot.2016.166 Authors: Philip R Nicovich, Dylan M Owen & Katharina Gaus Single-molecule localization microscopy (SMLM) generates super-resolution images by serially detecting individual fluorescent molecules. The power of SMLM ... mehr

Corrigendum: Interaction between RasV12 and scribbled clones induces tumour growth and invasion

Nature advance online publication 15 February 2017. doi:10.1038/nature21397 Author: Ming Wu, José Carlos Pastor-Pareja & Tian Xu mehr

A two-photon fluorescent probe for real-time monitoring autophagy by ultrasensitive detection of the change in lysosomal polarity

The first two-photon probe Lyso-OC for monitoring cell autophagy by detecting the change of lysosomal polarity during the membrane fusion process of autophagy was proposed. Lyso-OC exhibited desired optical properties and selective detection signal to the polarity change. More importantly, ... mehr

Alle Fachpublikationen

Overcoming spectral overlap via inductively coupled plasma – tandem mass spectrometry (ICP-MS/MS). A tutorial review.

This work reviews the operating principles of ICP – tandem mass spectrometry (ICP-MS/MS) and the key applications reported on since the introduction of the technique in 2012. The main differences between single quadrupole ICP-MS and ICP-MS/MS are elucidated and the tools available for metho ... mehr

Covalent immobilisation of antibodies in Teflon-FEP microfluidic devices for sensitive quantification of clinically relevant protein biomarkers

This study reports for the first time sensitive colorimetric and fluorescence detection of clinically relevant protein biomarkers by sandwich immunoassays using covalent immobilisation of antibodies onto the fluoropolymer surface inside Teflon®-FEP microfluidic devices. Teflon®-FEP has an o ... mehr

Investigation of the interactions between methylene blue and intramolecular G-quadruplexes: an explicit distinction in electrochemical behavior

G-quadruplex sequences exist in eukaryotic organisms and prokaryotes, and the investigation of interactions between G-quadruplexes and small molecule ligands is important for gene therapy, biosensor fabrication, fluorescence imaging and so on. Here, we investigated the behaviour of methylen ... mehr

Electrowetting without External Voltage using Paint-on Electrodes

Electrowetting uses voltage to manipulate small volumes of fluid for applications including lab-on-a-chip and optical devices. To avoid electrochemical reactions, a dielectric often separates the fluid from the electrode, which has the undesired effect of adding processing steps while incre ... mehr

Subcellular Probes for Neurochemical Recording from Multiple Brain Sites

Dysregulation of neurochemicals, in particular, dopamine, is epitomized in numerous debilitating disorders that impair normal movement and mood aspects of our everyday behavior. Neurochemical transmission is a neuron-specific process, and further exhibits region-specific signaling in the br ... mehr

Alle Fachpublikationen zu Analytik

A two-photon fluorescent probe for real-time monitoring autophagy by ultrasensitive detection of the change in lysosomal polarity

The first two-photon probe Lyso-OC for monitoring cell autophagy by detecting the change of lysosomal polarity during the membrane fusion process of autophagy was proposed. Lyso-OC exhibited desired optical properties and selective detection signal to the polarity change. More importantly, ... mehr

Fe3O4@ZIF-8: A Magnetic Nanocomposite for Highly Efficient UO2(2+) Adsorption and Selective UO2(2+)/Ln(3+) Separation

The magnetic nanocomposite Fe3O4@ZIF-8 has been firstly used as an excellent adsorbent for fast adsorption and seperation of UO2(2+) ions from aqueous solutions. This material exhibits an unprecedented adsorption capacity of 523.5 mg/g and remarkbale selectivity towards UO2(2+)/Ln3+ at pH = 3. mehr

Solvothermal Self-assembly of Cd2+ Coordination Polymers with Supramolecular Networks Involving N-donor Ligands and Aromatic Dicarboxylates: Synthesis, Crystal Structure and Ph ...

Two cadmium(II) coordination polymers (CPs) of compositions {[Cd(H2O)4(4-BPDB)][BPDC]}n (CP1) and {[Cd(H2O) (BrIP)(BTTMB)]·4MeOH}n (CP2) have been synthesized by solvothermal methods and characterized by several analytical tools including SXRD (Single Crystal X-ray Difraction). Structure of ... mehr

A novel strategy for high-performance transparent conductive films based on double-walled carbon nanotubes

High concentration of double-walled carbon nanotubes was dispersed by a carbazole-based conjugated polymer. And the transparent conductive film was fabricated via simple dip-coating using the dispersion, which showed simultaneously a low sheet resistance (average 134 Ω/□, minimal 86 Ω/□) an ... mehr

Chemical memory with states coded in light controlled oscillations of interacting Belousov–Zhabotinsky droplets

The information storing potential of droplets, in which an oscillatory, photosensitive Belousov–Zhabotinsky (BZ) reaction proceeds, is investigated experimentally. We consider coupled oscillations in pairs and triplets of droplets. Droplets are surrounded by a solution of lipids in decane. ... mehr

Alle Fachpublikationen zu Chemie

Selective deletion of adipocytes, but not preadipocytes, by TNF-|[alpha]| through C|[sol]|EBP- and PPAR|[gamma]|-mediated suppression of NF-|[kappa]|B

Selective deletion of adipocytes, but not preadipocytes, by TNF-α through C/EBP- and PPARγ-mediated suppression of NF-κB Laboratory Investigation 97, 228 (February 2017). doi:10.1038/labinvest.2016.124 Authors: Minori Tamai, Tsuyoshi Shimada, Nobuhiko Hiramatsu, Kunihi ... mehr

Inside the USCAP Journals

Inside the USCAP Journals Laboratory Investigation 97, 116 (February 2017). doi:10.1038/labinvest.2016.144 mehr

Malignant melanoma of sun-protected sites: a review of clinical, histological, and molecular features

Malignant melanoma of sun-protected sites: a review of clinical, histological, and molecular features Laboratory Investigation advance online publication, January 16 2017. doi:10.1038/labinvest.2016.147 Authors: Emily A Merkel & Pedram Gerami mehr

Unexpected UVR and non-UVR mutation burden in some acral and cutaneous melanomas

Unexpected UVR and non-UVR mutation burden in some acral and cutaneous melanomas Laboratory Investigation advance online publication, January 9 2017. doi:10.1038/labinvest.2016.143 Authors: Robert V Rawson, Peter A Johansson, Nicholas K Hayward, Nicola Waddell, Ann-Marie Patch ... mehr

The NF1 gene in tumor syndromes and melanoma

The NF1 gene in tumor syndromes and melanoma Laboratory Investigation advance online publication, January 9 2017. doi:10.1038/labinvest.2016.142 Authors: Maija Kiuru & Klaus J Busam mehr

Alle Fachpublikationen zu Labortechnik

Silo explosion from smoldering combustion: A case study

Abstract Smoldering combustion in power/dust deposits constitutes a significant problem in some process industries. Numerous bulk materials produce heat due to different biological and oxidation processes. Spontaneous heating occurs if the rate of heat generation by an exothermic proces ... mehr

Hydrogenation of m‐chloronitrobenzene over amorphous Ni‐B/CNTs catalysts: Promoting effect of CNTs confinement on the catalytic performance

ABSTRACT Ni‐B amorphous alloy particles were selectively loaded inside and outside of carbon nanotubes (CNTs) to form a series of Ni‐B/CNTs amorphous alloy catalysts. The confinement effects of the CNTs on the physicochemical properties of Ni‐B/CNTs were investigated. The results show t ... mehr

Adsorption of copper on glass beads coated with chitosan: Stirred batch and fixed bed analysis

ABSTRACT Chitosan is a natural polymer largely studied for heavy metal adsorption applications, since the amino and hydroxyl groups present in its structure can act as chelation sites. However, this material presents some drawbacks as adsorbent due to its low mechanical strength. Studie ... mehr

Reflection‐refraction effects on light distribution inside tubular photobioreactors

ABSTRACT One of the main parameters affecting autotrophic algae cultures is photon absorption distribution inside the photobioreactor. This clearly depends on the geometry of both the radiation source and the photobioreactor, as well as on algae suspension optical properties. In this wo ... mehr

Highly selective Fischer‐Tropsch synthesis for C10‐C20 diesel fuel under low pressure

ABSTRACT This work deals with the improvement of diesel (C10‐C20) production of Fischer‐Tropsch (FT) synthesis at low pressure over Co‐Re/Al2O3 since high catalytic activity and great potential in producing C10‐C20 hydrocarbons was observed under conventional conditions. To improve the ... mehr

Alle Fachpublikationen zu Verfahrenstechnik

Particle Size Specific Distribution of Perfluoro Alkyl Substances in Atmospheric Particulate Matter in Asian Cities

Seasonal and local characteristics of perfluorinated alkylated substances (PFASs) were examined using size segregated particles including an ultrafine range. The examination included sampling and analysis of ambient particles collected at four sites located in different environments in thre ... mehr

Sulfate Radical Oxidation of Aromatic Contaminants: A Detailed Assessment of Density Functional Theory and High-Level Quantum Chemical Methods

Advanced oxidation processes that utilize highly oxidative radicals are widely used in water reuse treatment. In recent years, the application of sulfate radical (SO4●-) as a promising oxidant for water treatment has gained increasing attention. To understand the efficiency of SO4●- in the ... mehr

Virulence Factor Activity Relationships (VFARs): A Bioinformatics Perspective

Virulence Factor Activity Relationships (VFAR) – a concept loosely based on quantitative structure-activity relationships (QSAR) for chemicals was proposed as a predictive tool for ranking risks due to microorganisms relevant to water safety. A rapid increase in sequencing capabilities and ... mehr

Unexpectedly high concentrations of monoterpenes in a study of UK homes

The abundance of volatile organic compounds (VOCs) found in homes depends on many factors such as emissions, ventilation and the oxidative environment and these are evolving over time, reflecting changes in chemical use, behaviour and building design/materials. The concentrations of VOCs in ... mehr

Prediction of Acute Toxicity of Emerging Contaminants on the Water Flea Daphnia magna by Ant Colony Optimization - Support Vector Machine QSTR models

According to the European REACH Directive, the acute toxicity towards Daphnia magna should be assessed for any industrial chemical with a market volume of more than 1 t/a. Therefore, it is highly recommended to determine the toxicity at a certain confidence level, either experimentally or b ... mehr

Alle Fachpublikationen zu Umwelttechnik
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