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Aktuelle Fachpublikationen

The structure of lipoyl synthase, a remarkable enzyme that performs the last step of an extraordinary biosynthetic pathway

Lipoic acid is assembled on its cognate proteins (e.g. the E2 subunit of pyruvate dehydrogenase). An octanoyl moiety is transferred from the octanoyl-ACP of fatty acid synthetase to a specific lysine residue of the cognate protein followed by sulfur insertion at C6 and C8 of the octanoyl ch ... mehr

The RED light is on! New tools for monitoring Ca2+ dynamics in the endoplasmic reticulum and mitochondria

In this issue of the Biochemical Journal, Wu et al. describe the characterization of new low-affinity Ca2+ indicators for monitoring Ca2+ levels in both the ER (endoplasmic reticulum) and mitochondria. In contrast with other ER Ca2+ sensors, these indicators emit in the red part of the spec ... mehr

Influenza A virus isolation, culture and identification

Nature Protocols 9, 2663 (2014). doi:10.1038/nprot.2014.180 Authors: Amie J Eisfeld, Gabriele Neumann & Yoshihiro Kawaoka Influenza A viruses (IAVs) cause epidemics and pandemics that result in considerable financial burden and loss of human life. To manage annual IAV epi ... mehr

Sodium Aspartate as a Specific Enhancer of Salty Taste Perception—Sodium Aspartate Is a Possible Candidate to Decrease Excessive Intake of Dietary Salt

The excessive intake of dietary salt is a global issue in health. Attempts have been made to address this issue, including the development of salt substitutes. Yet, none of these substances are currently in wide use, because of their weak saltiness. The purpose of this study was to assess t ... mehr

Gray Matter Volume Reduction of Olfactory Cortices in Patients With Idiopathic Olfactory Loss

Idiopathic olfactory loss (IOL) is a common olfactory disorder. Little is known about the pathophysiology of this disease. Previous studies demonstrated decreased olfactory bulb (OB) volume in IOL patients when compared with controls. The aim of our study was to investigate structural brain ... mehr

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Multiplex chemiluminescent biosensor for type B-fumonisins and aflatoxin B1 quantitative detection in maize flour

A multiplex chemiluminescent biosensor for simple, rapid and ultrasensititive on-site quantification of Aflatoxin B1 and type B-Fumonisins in maize samples has been developed. The biosensor integrates a multiplex indirect competitive lateral flow immunoassay (LFIA) based on enzyme-catalyzed ... mehr

Graphene Oxide Amplified Fluorescence Anisotropy for Label-Free Detection of Potassium Ion

Fluorescence anisotropy (FA) has attracted a lot of attentions, but it has been scarely applied for the detection of small molecules and metal ions because they are too small to induce obvious FA changes. Although some mass amplifying strategies have been developed, the recognition probes n ... mehr

Cell proliferation and migration inside single cell arrays

Cell proliferation and migration are fundamental processes in determining cell and tissue behaviour. In this study we show the design and fabrication of a new single cell microfluidic structure, called a “vertically integrated array” or “VIA” trap to explore quantitative functional assays i ... mehr

Microfluidic in situ mechanical testing of photopolymerized gels

Gels are a functional template for micro-particle fabrication and microbiology experiments. The control and knowledge of their mechanical properties is critical in a number of applications, but no simple in situ method exists to determine these properties. We propose a novel microfluidic ba ... mehr

Real-time microfluidic recombinase polymerase amplification for the toxin B gene of Clostridium difficile on a SlipChip platform

Clostridium difficile is one of the key bacterial pathogens that cause infectious diarrhoea both in the developed and developing world. Isothermal nucleic acid amplification methods are increasingly used for identification of toxinogenic infection by clinical labs. For this purpose, we deve ... mehr

Alle Fachpublikationen zu Analytik

The structure of lipoyl synthase, a remarkable enzyme that performs the last step of an extraordinary biosynthetic pathway

Lipoic acid is assembled on its cognate proteins (e.g. the E2 subunit of pyruvate dehydrogenase). An octanoyl moiety is transferred from the octanoyl-ACP of fatty acid synthetase to a specific lysine residue of the cognate protein followed by sulfur insertion at C6 and C8 of the octanoyl ch ... mehr

The RED light is on! New tools for monitoring Ca2+ dynamics in the endoplasmic reticulum and mitochondria

In this issue of the Biochemical Journal, Wu et al. describe the characterization of new low-affinity Ca2+ indicators for monitoring Ca2+ levels in both the ER (endoplasmic reticulum) and mitochondria. In contrast with other ER Ca2+ sensors, these indicators emit in the red part of the spec ... mehr

Sodium Aspartate as a Specific Enhancer of Salty Taste Perception—Sodium Aspartate Is a Possible Candidate to Decrease Excessive Intake of Dietary Salt

The excessive intake of dietary salt is a global issue in health. Attempts have been made to address this issue, including the development of salt substitutes. Yet, none of these substances are currently in wide use, because of their weak saltiness. The purpose of this study was to assess t ... mehr

Gray Matter Volume Reduction of Olfactory Cortices in Patients With Idiopathic Olfactory Loss

Idiopathic olfactory loss (IOL) is a common olfactory disorder. Little is known about the pathophysiology of this disease. Previous studies demonstrated decreased olfactory bulb (OB) volume in IOL patients when compared with controls. The aim of our study was to investigate structural brain ... mehr

A Determinant of Odorant Specificity Is Located at the Extracellular Loop 2-Transmembrane Domain 4 Interface of an Anopheles gambiae Odorant Receptor Subunit

To explore the structural basis for odorant specificity in odorant receptors of the human malaria vector mosquito, Anopheles gambiae, odorant-binding subunits (Agam\Ors) expressed in Xenopus oocytes in combination with Agam\Orco (coreceptor subunit) were assayed by 2-electrode voltage clamp ... mehr

Alle Fachpublikationen zu Chemie

Fibroblasts induce expression of FGF4 in ovarian cancer stem-like cells/cancer-initiating cells and upregulate their tumor initiation capacity

Fibroblasts induce expression of FGF4 in ovarian cancer stem-like cells/cancer-initiating cells and upregulate their tumor initiation capacity Laboratory Investigation advance online publication, October 20 2014. doi:10.1038/labinvest.2014.122 Authors: Kazuyo Yasuda, Toshihiko ... mehr

Immortalized multipotent pericytes derived from the vasa vasorum in the injured vasculature. A cellular tool for studies of vascular remodeling and regeneration

Immortalized multipotent pericytes derived from the vasa vasorum in the injured vasculature. A cellular tool for studies of vascular remodeling and regeneration Laboratory Investigation advance online publication, October 20 2014. doi:10.1038/labinvest.2014.121 Authors: Maki K ... mehr

Inside the USCAP Journals

Inside the USCAP Journals Laboratory Investigation 94, 936 (September 2014). doi:10.1038/labinvest.2014.106 mehr

Inside the USCAP Journals

Inside the USCAP Journals Laboratory Investigation 94, 820 (August 2014). doi:10.1038/labinvest.2014.88 mehr

Inside the USCAP Journals

Inside the USCAP Journals Laboratory Investigation 94, 704 (July 2014). doi:10.1038/labinvest.2014.74 mehr

Alle Fachpublikationen zu Labortechnik

De‐SOX Performance of Spinels Containing Manganese

Abstract The development of sulfur transfer agents plays a significant role in the environmental pollution control. However, at present, there are only few researches on the addition of active components to the sulfur transfer agent. Using manganese as an active component, the structure ... mehr

Cover Chem. Eng. Technol. 11/2014

Abstract Tank equipment. Copyright: xmagics – Fotolia.com mehr

Design and optimization of an extrusion die for the production of wood–plastic composite profiles

In this work, the optimization of an extrusion die designed for the production of a wood–plastic composite (WPC) decking profile is investigated. The optimization was performed with the help of numerical tools, more precisely, by solving the continuity and momentum conservation equations th ... mehr

Numerical modeling of multilayer film coextrusion with experimental validation

Computational fluid dynamics (CFD) using a finite volume technique and the volume of fluid method of interface tracking is used to model the production of polyester‐based multilayered films via coextrusion. Experimental methods encompass both overall flow validation and secondary layer thic ... mehr

Microwave processing of syntactic foam from an expandable thermoset/thermoplastic mixture

In this work, a microwave expansion process to produce thermoset‐matrix syntactic foam containing thermoplastic foam beads was designed and developed. Expandable polystyrene (EPS) microspheres and epoxy resin were chosen as a model material system. This process is featured with a capability ... mehr

Alle Fachpublikationen zu Verfahrenstechnik

Microbial electrolysis cell accelerates phosphate remobilisation from iron phosphate contained in sewage sludge

Phosphate was remobilised from iron phosphate contained in digested sewage sludge with a bio-electric cell. A 500 fold acceleration above former results was caused by strongly basic catholytes. For these experiments a dual chambered microbial electrolysis cell with a small cathode (40 mL) a ... mehr

Acesulfame-K and pharmaceuticals as co-tracers of municipal wastewater in a receiving river

Wastewater treatment plant (WWTP) effluents are important sources of emerging contaminants at environmentally-relevant concentrations. In this study, water samples were collected from a river downstream of two WWTPs to identify practical tracers for tracking wastewater. The results of the s ... mehr

Effect of permafrost property on gas hydrate petroleum system in the Qilian Mountain, Qinghai, Northwest China

The gas hydrate petroleum system in the Qilian Mountain permafrost, which exists as an epigenetic hydrocarbon reservoir situated over a deep-seated potential hydrocarbon reservoir, is dynamic since the end of the late Pleistocene due to climate change. The permafrost property not only can l ... mehr

Biological effects and bioaccumulation of pharmaceutically active compounds in crucian carp caged near the outfall of a sewage treatment plant

Pharmaceutically active compounds (PhACs) have been universally detected in rivers, lakes and coastal waters affected by effluents from sewage treatment plants (STPs). In this study, the biological effects and bioaccumulation of PhACs were assessed in crucian carp (Carassius auratus) caged ... mehr

Correction: Statistical modeling of crystalline silica exposure by trade in the construction industry using a database compiled from the literature

Correction for ‘Statistical modeling of crystalline silica exposure by trade in the construction industry using a database compiled from the literature’ by Jean-François Sauvé et al., J. Environ. Monit., 2012, 14, 2512–2520. mehr

Alle Fachpublikationen zu Umwelttechnik
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