Meine Merkliste
my.chemie.de  
Login  
FACHPUBLIKATIONEN

Fachpublikationen

Suchen Sie Fachveröffentlichungen anhand ihres Titels oder Autors und präzisieren Sie Ihre Suche danach anhand von Verlagen, Jahrgängen usw.

Aktuelle Fachpublikationen

CRISPR/Cas9-mediated conversion of eGFP- into Gal4-transgenic lines in zebrafish

Nature Protocols 9, 2823 (2014). doi:10.1038/nprot.2014.187 Authors: Thomas O Auer, Karine Duroure, Jean-Paul Concordet & Filippo Del Bene Here we present a protocol for the conversion of eGFP-transgenic zebrafish lines into lines expressing Gal4 from the same locus. This ... mehr

The crystal structure of the RhoA-AKAP-Lbc DH-PH domain complex

The RhoGEF (Rho GTPase guanine-nucleotide-exchange factor) domain of AKAP-Lbc (A-kinase-anchoring protein-Lbc, also known as AKAP13) catalyses nucleotide exchange on RhoA and is involved in the development of cardiac hypertrophy. The RhoGEF activity of AKAP-Lbc has also been implicated in c ... mehr

Cinderella finds her shoe: the first Vps34 inhibitor uncovers a new PI3K-AGC protein kinase connection

Class II/III PI3Ks (phosphoinositide 3-kinases) produce the PtdIns(3)P lipid that is involved in intracellular vesicular trafficking. In contrast with class I PI3Ks, the potential signalling roles of class II/III PI3Ks are poorly understood. In a recent article in the Biochemical Journal, B ... mehr

Antibody structural modeling with prediction of immunoglobulin structure (PIGS)

Nature Protocols 9, 2771 (2014). doi:10.1038/nprot.2014.189 Authors: Paolo Marcatili, Pier Paolo Olimpieri, Anna Chailyan & Anna Tramontano Antibodies (or immunoglobulins) are crucial for defending organisms from pathogens, but they are also key players in many medical, d ... mehr

3D spheroid model of mIMCD3 cells for studying ciliopathies and renal epithelial disorders

Nature Protocols 9, 2725 (2014). doi:10.1038/nprot.2014.181 Authors: Rachel H Giles, Henry Ajzenberg & Peter K Jackson We have developed a novel 3D cell culture model that uses mouse inner-medullary collecting duct (mIMCD3) cells to generate epithelial spheroids. This mod ... mehr

Alle Fachpublikationen

A trial study on proteomics fingerprint analysis of HepaRG cells by FD-LC-MS/MS

A proteomics profile analysis was performed on a human hepatocyte carcinoma cell line (HepaRG) using the FD-LC-MS/MS method. One hundred fifty eight proteins were identified for the first time and 10 were specific to human hepatocytes. These proteins are a “proteomics fingerprint” that can ... mehr

Fabrication of fluorescent SiO2@ zeolitic imidazolate framework-8 nanosensor for Cu2+ detection

A simple strategy to fabricate fluorescent SiO2@zeolitic imidazolate framework-8 (ZIF-8) core-shell nanosensor for Cu2+ detection was demonstrated in this work. The nanosensor was synthesized using carboxyl-functionalized SiO2 nanoparticles (SiO2 NPs) as a template to induce the growth of Z ... mehr

Probing the Secondary Structure of Bovine Serum Albumin during Heat-Induced Denaturation Using Mid-Infrared Fiberoptic Sensors

Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy using a special waveguide based on a silver halide fiber was used for probing the heat-induced secondary structure and conformation changes of bovine serum albumin (BSA). From the secondary derivative and the cur ... mehr

A Neutrophil Treadmill to Decouple Spatial and Temporal Signals during Chemotaxis

After more than 50 years of debates, the role of spatial and temporal gradients during cell chemotaxis is still a contentious matter. One major challenge is that when cells move in response to a heterogeneous chemical environment they are exposed to both spatial and temporal concentration c ... mehr

Dual-functional probes for sequential thiol and redox homeostasis sensing in live cells

A new type of resorufin-based dual-functional fluorescent probe whose fluorescence emission features are sensitive to thiol compounds and redox homeostasis was developed. Thiols-triggered nucleophilic substitution of the probes converts the nonfluorescent probe to the highly fluorescent res ... mehr

Alle Fachpublikationen zu Analytik

The crystal structure of the RhoA-AKAP-Lbc DH-PH domain complex

The RhoGEF (Rho GTPase guanine-nucleotide-exchange factor) domain of AKAP-Lbc (A-kinase-anchoring protein-Lbc, also known as AKAP13) catalyses nucleotide exchange on RhoA and is involved in the development of cardiac hypertrophy. The RhoGEF activity of AKAP-Lbc has also been implicated in c ... mehr

Cinderella finds her shoe: the first Vps34 inhibitor uncovers a new PI3K-AGC protein kinase connection

Class II/III PI3Ks (phosphoinositide 3-kinases) produce the PtdIns(3)P lipid that is involved in intracellular vesicular trafficking. In contrast with class I PI3Ks, the potential signalling roles of class II/III PI3Ks are poorly understood. In a recent article in the Biochemical Journal, B ... mehr

Carbon quantum dot–NO photoreleaser nanohybrids for two-photon phototherapy of hypoxic tumors

We report a conjugate between carbon quantum dots and a NO photoreleaser able to photogenerate the anticancer NO radical via an energy transfer mechanism. This nanohybrid proved toxic to cancer cells in vitro and significantly reduced tumor volume in mice bearing human xenograft BxPC-3 panc ... mehr

Binding of Ru(terpyridine)(pyridine)dipyridophenazine to DNA studied with polarized spectroscopy and calorimetry

Linear and circular dichroism (LD and CD) spectroscopy as well as isothermal titration calorimetry (ITC) have been used to investigate the interaction of Ru(tpy)(py)dppz2+ (tpy = 2,2′:6′,2′′-terpyridyl; py = pyridine; dppz = dipyrido[3,2-a:2′3′-c]phenazine) with DNA, providing detailed info ... mehr

A highly efficient and eco-friendly method for the synthesis of 1,3-indandione ring-fused 3-oxindoles bearing two contiguous quaternary stereocenters via an aldol reaction in a ...

A highly efficient and environmentally benign method for the synthesis of oxindoles featuring two contiguous quaternary carbon centers via an aldol reaction starting from various 3-substituted oxindoles has been established. A wide variety of such featured multi-substituted 1,3-indandione r ... mehr

Alle Fachpublikationen zu Chemie

Development of non-traumatic osteonecrosis of the femoral head requires toll-like receptor 7 and 9 stimulations and is boosted by repression on nuclear factor kappa B in rats

Development of non-traumatic osteonecrosis of the femoral head requires toll-like receptor 7 and 9 stimulations and is boosted by repression on nuclear factor kappa B in rats Laboratory Investigation advance online publication, November 10 2014. doi:10.1038/labinvest.2014.134 ... mehr

Inside the USCAP Journals

Inside the USCAP Journals Laboratory Investigation 94, 1186 (November 2014). doi:10.1038/labinvest.2014.127 mehr

Fibroblasts induce expression of FGF4 in ovarian cancer stem-like cells/cancer-initiating cells and upregulate their tumor initiation capacity

Fibroblasts induce expression of FGF4 in ovarian cancer stem-like cells/cancer-initiating cells and upregulate their tumor initiation capacity Laboratory Investigation advance online publication, October 20 2014. doi:10.1038/labinvest.2014.122 Authors: Kazuyo Yasuda, Toshihiko ... mehr

Immortalized multipotent pericytes derived from the vasa vasorum in the injured vasculature. A cellular tool for studies of vascular remodeling and regeneration

Immortalized multipotent pericytes derived from the vasa vasorum in the injured vasculature. A cellular tool for studies of vascular remodeling and regeneration Laboratory Investigation advance online publication, October 20 2014. doi:10.1038/labinvest.2014.121 Authors: Maki K ... mehr

Inside the USCAP Journals

Inside the USCAP Journals Laboratory Investigation 94, 936 (September 2014). doi:10.1038/labinvest.2014.106 mehr

Alle Fachpublikationen zu Labortechnik

Morphology and rheological properties of silica‐filled poly(carbonate)/poly(methyl methacrylate) blends

The effect of silica nanoparticles on the morphology and the rheological properties was investigated in the immiscible polymer blend poly(carbonate)/poly(methyl methacrylate) (PC/PMMA). In the melt state, the linear viscoelastic properties of the nanocomposite showed a reduction effect of t ... mehr

Extrusion film casting of long chain branched polypropylene

Extrusion film casting (EFC) is an important melt processing operation which is extensively used to make polypropylene (PP) films. Linear PP shows significant amount of necking and draw resonance during EFC. One of the ways to reduce necking is to introduce long chain branches (LCB) on the ... mehr

Influence of the drying route on the depolymerization and properties of chitosan

In this work, a chitosan sample with a high degree of deacetylation (DD >95%) obtained from freshwater shrimp shells was subjected to drying processes in an electric oven and by supercritical CO2. The results indicated that drying chitosan particles with supercritical CO2 resulted in a very ... mehr

A Comprehensive Kinetic Study of the Reaction of SO2 with CaO by the Random Pore Model

Abstract The kinetics of the reaction between CaO and SO2 was studied by the random pore model, to accurately consider the porous structure of the solid reactant. The inherent reaction rate constants and rate dependency with respect to the SO2 concentration were determined from the initi ... mehr

Formation of molecular nitrogen and hydrogen sulfide during high‐temperature pyrolysis of coals

ABSTRACT Nitrogen and sulfur release from eleven coals during pyrolysis at high temperatures (≥1000 °C) was studied in both fixed‐bed and free‐fall quartz reactors. Yields of N2, char‐N and H2S were found to depend strongly on the pyrolysis conditions, such that N2 and H2S yields increas ... mehr

Alle Fachpublikationen zu Verfahrenstechnik

Emerging pollutants in the Esmeraldas watershed in Ecuador: discharge and attenuation of emerging organic pollutants along the San Pedro–Guayllabamba–Esmeraldas rivers

Water quality characteristics and emerging organic pollutants were sampled along the San Pedro–Guayllabamba–Esmeraldas River and its main water pollution streams in the summer of 2013. The annual flow rate of the stream is 22 [ ] 000 Mm3 y−1 and it collects the wastewater of Quito-Ecuador i ... mehr

Atmospheric 210Pb as a tracer for soil organic carbon transport in a coniferous forest

Core soils and falling litter samples were collected in Japanese cypress forest (Chamaecyparis obtuse) to determine the litter-feed 210Pbex and organic carbon transfer from forest canopy to soil and their subsequent distribution. Of the canopy residing 210Pbex pool, litterfall annually tran ... mehr

Biological effects and bioaccumulation of pharmaceutically active compounds in crucian carp caged near the outfall of a sewage treatment plant

Pharmaceutically active compounds (PhACs) have been universally detected in rivers, lakes and coastal waters that are affected by effluents from sewage treatment plants (STPs). In this study, the biological effects and bioaccumulation of PhACs were assessed in crucian carp (Carassius auratu ... mehr

Chelation Technology: A Promising Green Approach for Resource Management and Waste Minimization.

Green chemical engineering perceives the concept of developing innovative environmental benign technologies to protect human health and ecosystem. In order to explore this concept for minimizing the industrial waste and for reducing the environmental impact of hazardous chemicals, new green ... mehr

An evaluation of the “GGP” personal samplers under semi-volatile aerosols: sampling losses and their implication on occupational risk assessment

Semi-volatile (SV) aerosols still represent an important challenge to occupational hygienists due to toxicological and sampling issues. Particularly problematic is the sampling of hazardous SV that are present in both particulate and vapour phases at a workplace. In this study we investigat ... mehr

Alle Fachpublikationen zu Umwelttechnik
Ihr Bowser ist nicht aktuell. Microsoft Internet Explorer 6.0 unterstützt einige Funktionen auf Chemie.DE nicht.