10.04.2013 | Terence G Henares; Yuta Uenoyama; Yuto Nogawa; Ken Ikegami; Daniel Citterio; Koji Suzuki; Shun-ichi Funano; Kenji Su ..., Analyst, 2013

This paper presents a novel rhodamine diphosphate molecule that allows highly-sensitive detection of proteins by employing sequential enzyme-linked immunosorbent assay and capillary isoelectric focusing (ELISA-cIEF). Seven-fold improvement in the immunoassay sensitivity and a 1-2 order of ...

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10.04.2013 | Seung-A Lee; Da Yoon No; Edward Kang; JongIl Ju; Dong-Sik Kim; SangHoon Lee, Lab on a Chip, 2013

We have developed a three-dimensional (3D) liver-on-a-chip to investigate the interaction of hepatocytes and hepatic stellate cells (HSCs) in which primary 3D hepatocyte spheroids and HSCs are co-cultured without direct cell-cell contact. Here, we show that the 3D liver chip offers ...

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10.04.2013 | Ansgar Waldbaur; Jörg Kittelmann; Carsten Philipp Radtke; Jürgen Hubbuch; Bastian E. Rapp, Lab on a Chip, 2013

We describe a generic microfluidic interface design that allows connecting microfluidic chips to established industrial liquid handling stations (LHS). A moulding tool has been designed that allows fabrication of low-cost disposable polydimethylsiloxane (PDMS) chips with interfaces that ...

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10.04.2013 | Lin Tang; Yaoyu Zhou; Guangming Zeng; Zhen Li; Yuanyuan Liu; Yi Zhang; Guiqiu Chen; Guide Yang; Xiaoxia Lei; Mengshi Wu, Analyst, 2013

A novel biosensor was developed based on tyrosinase immobilization with ordered mesoporous carbon-Au (OMC-Au), L-Lysine membrane and Au nanoparticles on glassy carbon electrode (GCE), and applied for simultaneous determination of dihydroxybenzene isomers using the differential pulse ...

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10.04.2013 | Fang Liu; Heng Liu; Meng Zhang; Jinghua Yu; Shaowei Wang; Juanjuan Lu, Analyst, 2013

Aiming at the facile detection of lengthy DNA molecules, an easily-operated sandwich-type electrochemiluminescence (ECL) DNA biosensor was constructed on glassy carbon electrode (GCE) based on CdTe quantum dots coated hollow ZnO nanoparticles (CdTe/ZnO NPs)-S2O82- ECL system in this work. To ...

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10.04.2013 | Luigi Sasso; Arto Heiskanen; Francesco Diazzi; Maria Dimaki; Jaime Castillo; Marco Vergani; Ettore Landini; Roberto ..., Analyst, 2013

A surface modification of interdigitated gold microelectrodes (IDEs) with a doped polypyrrole (PPy) film for detection of dopamine released from populations of differentiated PC12 cells is presented. A thin PPy layer was potentiostatically electropolymerized from an aqueous pyrrole solution ...

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10.04.2013 | Todd A Duncombe; Amy Herr, Lab on a Chip, 2013

Designed for compatibility with slab-gel polyacrylamide gel electrophoresis (PAGE) reagents and instruments, we detail development of free-standing polyacrylamide gel (fsPAG) microstructures supporting electrophoretic performance rivalling that of microfluidic platforms. For the protein ...

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10.04.2013 | Cassandra Sekol Niman; Jason Paul Beech; Jonas O Tegenfeldt; Paul M.G. Curmi; Derek Neil Woolfson; Nancy R Forde; He ..., Lab on a Chip, 2013

The ability to test the response of cells and proteins to a changing biochemical environment is of interest for studies of fundamental cell physiology and molecular interactions. In a common experimental scheme the cells or molecules of interest are attached to a surface and the composition ...

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10.04.2013 | Zhenyu Lin; Guonan Chen, Analyst, 2013

A simple, cost-efficient, sensitive and selective fluorescence sensor is developed for sequence-specific recognition of duplex DNA (ds-DNA) in vitro using H2dtoaCu (N,N’-bis(2-hydroxyethyl)dithiooxamidato copper (II)) as the sensing platform. H2dtoaCu can strongly chemisorb the dye-labeled ...

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10.04.2013 | Robert Penchovsky, Lab on a Chip, 2013

Advances in modern genomic research depend heavily on applications of various devices for automated high- or ultra-throughput arrays. Micro- and nanofluidics offer possibilities for miniaturization and integration of many different arrays onto a single device. Therefore, such devices are ...

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