01.04.2013 | Palacios, A. G., Lee, T. M., CSH Protocols, 2013

The Octodon degu is a native rodent species from South America, which lives in colonies with a well-structured social organization grouping of 5–10 young and 2–5 adult animals sharing a burrow system. They show a temperature-dependent diurnal-crepuscular activity pattern. In nature they ...

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01.04.2013 | Nilsen, T. W., CSH Protocols, 2013

Determining RNA structures (i.e., single- and double-strand regions) is often useful when assessing the potential for certain RNAs to interact with proteins or when determining whether RNAs that are dissimilar in sequence can form the same structure. A number of ribonucleases (RNases) have ...

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01.04.2013 | Thomas, R. C., Bers, D. M., CSH Protocols, 2013

Ca2+-sensitive microelectrodes (CaSMs) directly measure the pCa at their tip, which can be in a small extracellular space or inside a large and robust cell. They do not add to buffering and do not require expensive equipment. But they are time-consuming to make, require a reference electrode ...

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01.04.2013 | Ostrovsky, A., Cachero, S., Jefferis, G., CSH Protocols, 2013

Clonal analysis with the MARCM (mosaic analysis with a repressible cell marker) system can be used for studying cell lineage, development, and anatomy in the Drosophila olfactory system and other parts of the fly brain. To compare confocal images of labeled neurons in different brains, it ...

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01.04.2013 | Ostrovsky, A., Cachero, S., Jefferis, G., CSH Protocols, 2013

Clonal analysis with the MARCM (mosaic analysis with a repressible cell marker) system can be used for studying cell lineage, development, and anatomy in the Drosophila olfactory system and other parts of the fly brain. This protocol describes the dissection, staining, and imaging of brains ...

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01.04.2013 | Amber R. English; Gia K. Voeltz, CSH Perspectives, 2013

The endoplasmic reticulum (ER) is a large, continuous membrane-bound organelle comprised of functionally and structurally distinct domains including the nuclear envelope, peripheral tubular ER, peripheral cisternae, and numerous membrane contact sites at the plasma membrane, mitochondria, ...

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01.04.2013 | Kim, H. Y., Davidson, L. A., CSH Protocols, 2013

To understand dynamic cell and tissue mechanics during Xenopus laevis morphogenesis requires the use of live reporters of cell architecture as well as reporters for the location and activity of proteins in cells within a multicellular tissue. Fluorescently tagged proteins are some of the ...

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01.04.2013 | David R. McIlwain; Thorsten Berger; Tak W. Mak, CSH Perspectives, 2013

Caspases are a family of endoproteases that provide critical links in cell regulatory networks controlling inflammation and cell death. The activation of these enzymes is tightly controlled by their production as inactive zymogens that gain catalytic activity following signaling events ...

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01.04.2013 | Ostrovsky, A., Cachero, S., Jefferis, G., CSH Protocols, 2013

Clonal analysis with the MARCM (mosaic analysis with a repressible cell marker) system can be used for studying cell lineage, development, and anatomy in the Drosophila olfactory system and other parts of the fly brain. This protocol gives a method for generating flies with mosaic labeling. ...

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01.04.2013 | Jeffrey D. Stumpf; Russell P. Saneto; William C. Copeland, CSH Perspectives, 2013

The inability to replicate mitochondrial genomes (mtDNA) by the mitochondrial DNA polymerase (pol ) leads to a subset of mitochondrial diseases. Many mutations in POLG, the gene that encodes pol , have been associated with mitochondrial diseases such as myocerebrohepatopathy spectrum (MCHS) ...

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