01.04.2013 | Takashi Sawai; Akihisa Kamataki; Miwa Uzuki; Kinji Ishida; Tomohito Hanasaka; Kensuke Ochi; Takahito Hashimoto; Taka ..., Journal of Electron Microscopy, 2013

To evaluate the advantages of combination of two advanced electron microscopic technologies such as serial block-face scanning electron microscopy and double-axis electron beam tomography, we analyzed the three-dimensional morphology of cellular relationships between dendritic and plasma ...

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01.04.2013 | Ryusuke Sagawa; Wataru Togashi; Tomoki Akita; Yoshizo Takai, Journal of Electron Microscopy, 2013

The crystal structure of a new type of molybdenum oxide crystal encapsulated in a single-walled carbon nanotube (CNT) was examined via diffraction and spectroscopic techniques using both X-rays and electron beams. This new type of molybdenum oxide crystal has a chemical bonding state of ...

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01.04.2013 | Ii-sei Watanabe; Fernando José Dias; João Paulo Mardegan Issa; Carlos Alexandre dos Santos Haemmerle; Diego Pulzatto ..., Journal of Electron Microscopy, 2013

The sensory nerve endings of the rat tongue, cheek and palate were studied using immunohistochemical staining and transmission electron microscopy analysis. The specimens were fixed in modified Karnovsky solution and embedded in Spurr resin. Substance P, calcitonin gene-related peptide ...

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01.04.2013 | Masanori Mitome, Journal of Electron Microscopy, 2013

The low-energy Ar-ion milling method was used to prepare ultrathin specimens for transmission electron microscope observation. The samples were thinned initially by a usual focused ion beam technique or typical Ar-ion milling with a high energy of 2–10 keV and were thinned additionally by an ...

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01.04.2013 | Marie Kopecká; Susumu Kawamoto; Masashi Yamaguchi, Journal of Electron Microscopy, 2013

The F-actin cytoskeleton of Cryptococcus neoformans is known to comprise actin cables, cortical patches and cytokinetic ring. Here, we describe a new F-actin structure in fungi, a perinuclear F-actin collar ring around the cell nucleus, by fluorescent microscopic imaging of rhodamine ...

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01.04.2013 | Masashi Watanabe, Journal of Electron Microscopy, 2013

For development of advanced materials, characterization using a scanning transmission electron microscope (STEM) including analysis via X-ray energy-dispersive spectrometry and electron energy-loss spectrometry is essential. Recent advances in aberration-corrected instruments have offered ...

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01.04.2013 | Masaaki Kawata; Chikara Sato, Journal of Electron Microscopy, 2013

Data mining from noisy data/images is one of the most important themes in modern science and technology. Statistical image processing is a promising technique for analysing such data. Automation of particle pickup from noisy electron micrographs is essential, especially when improvement of ...

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01.04.2013 | Christian Ricolleau; Jaysen Nelayah; Tetsuo Oikawa; Yuji Kohno; Nadi Braidy; Guillaulle Wang; Florian Hue; Lenuta Fl ..., Journal of Electron Microscopy, 2013

The performances of a newly developed 80–200 kV cold field emission gun (CFEG) transmission electron microscope (TEM) integrating a spherical aberration corrector for a TEM image-forming lens have been evaluated. To begin, we show that the stability of both emission and probe currents makes ...

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01.04.2013 | Hiroshi Jinnai; Toshihiko Tsuchiya; Sohei Motoki; Takeshi Kaneko; Takeshi Higuchi; Atsushi Takahara, Journal of Electron Microscopy, 2013

This review summarizes the recent advances in three-dimensional (3D) imaging techniques and their application to polymer nanostructures, for example, microphase-separated structures of block copolymers. We place particular emphasis on the method of transmission electron microtomography ...

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01.04.2013 | Takeshi Matsubara; Yuka Akiyama; Kenzi Oshima; Tetsuya Okajima; Daita Nadano; Tsukasa Matsuda, Journal of Biochemistry, 2013

Allergenic potential of food proteins is associated with stability to gastric and pancreatic digestive enzymes. However, much attention has not been focused on intracellular digestion of protein antigens during the passage through intestinal epithelia. We report here the degradation and ...

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