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10.435 Aktuelle Fachpublikationen in Analytical Chemistry

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3D Network and 2D Paper of Reduced Graphene Oxide/Cu2O Composite for Electrochemical Sensing of Hydrogen Peroxide

19.01.2018 | Chunfeng Cheng; Chunmei Zhang; Xiaohui Gao; Zhihua Zhuang; Cheng Du; Wei Chen, Analytical Chemistry, 2018

In this study, two-dimensional (2D) and three-dimensional (3D) freestanding reduced graphene oxide-supported Cu2O composites (Cu2O-rGO) were synthesized via simple and cost-efficient hydrothermal and filtration strategies. The structural characterizations clearly showed that highly porous 3D ...

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High Initial Sputter Rate Found for Vaccinia Virions Using Isotopic Labeling, NanoSIMS, and AFM

19.01.2018 | Sean D. Gates; Richard C. Condit; Nissin Moussatche; Benjamin J. Stewart; Alexander J. Malkin; Peter K. Weber, Analytical Chemistry, 2018

High-lateral-resolution secondary ion mass spectrometry (SIMS) has the potential to provide functional and depth resolved information from small biological structures, such as viral particles (virions) and phage, but sputter rate and sensitivity are not characterized at shallow depths relevant to ...

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Improving Mass Spectral Quality of Monoclonal Antibody Middle-Up LC-MS Analysis by Shifting the Protein Charge State Distribution

19.01.2018 | Wei Ding; Difei Qiu; Mark S. Bolgar; Scott A. Miller, Analytical Chemistry, 2018

Monoclonal antibodies (mAbs) are experiencing accelerated development in the pharmaceutical industry. Utilization of middle-up LC-MS methodology can provide detailed characterization of mAbs via reduction and/or enzymatic cleavage of the mAb into smaller protein fragments. However, under typical ...

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Highly Accurate Quantitative Analysis Of Enantiomeric Mixtures from Spatially Frequency Encoded 1H NMR Spectra

19.01.2018 | Bertrand Plainchont; Daisy Pitoux; Mathieu Cyrille; Nicolas Giraud, Analytical Chemistry, 2018

We propose an original concept to measure accurately enantiomeric excesses on proton NMR spectra, which combines high-resolution techniques based on a spatial encoding of the sample, with the use of optically active weakly orienting solvents. We show that it is possible to simulate accurately ...

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Aptamer-Based Dual-Functional Probe for Rapid and Specific Counting and Imaging of MCF-7 Cells

19.01.2018 | Bin Yang; Beibei Chen; Man He; Xiao Yin; Chi Xu; Bin Hu, Analytical Chemistry, 2018

Development of multimodal detection technologies for accurate diagnosis of cancer at early stages is in great demand. In this work, we report a novel approach using an aptamer-based dual-functional probe for rapid, sensitive, and specific counting and visualization of MCF-7 cells by inductively ...

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Identification of Individual Bacterial Cells through the Intermolecular Interactions with Peptide-Functionalized Solid-State Pores

19.01.2018 | Makusu Tsutsui; Masayoshi Tanaka; Takahiro Marui; Kazumichi Yokota; Takeshi Yoshida; Akihide Arima; Wataru Tonomura; ..., Analytical Chemistry, 2018

Bioinspired pore sensing for selective detection of flagellated bacteria was investigated. The Au micropore wall surface was modified with a synthetic peptide designed from toll-like receptor 5 (TLR5) to mimic the pathogen-recognition capability. We found that intermolecular interactions between ...

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Detection of Impurities in Organic Crystals by High-Accuracy Terahertz Absorption Spectroscopy

19.01.2018 | Tetsuo Sasaki; Tomoaki Sakamoto; Makoto Otsuka, Analytical Chemistry, 2018

Quantitative detection of impurities in organic crystals was demonstrated by accurately measuring absorption frequencies using a continuous wave gallium phosphide terahertz spectrometer. THz spectra of l-asparagine monohydrate doped with l-aspartic acid at 0.05 to 12.5 wt % were obtained at 10 K. ...

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Use of Cysteine Aminoethylation To Identify the Hypervariable Peptides of an Antibody

19.01.2018 | Nick DeGraan-Weber; James Patrick Reilly, Analytical Chemistry, 2018

Aminoethylation of cysteines can provide enzymatically cleavable sites. The ability to obtain peptides containing antibody complementarity determining regions (CDRs) with aminoethylated cysteines was investigated. Because cysteines are often located N-terminal to CDRs, digestion with Lys-N ...

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Correction to Fingerprint Analysis: Moving Toward Multiattribute Determination via Individual Markers

18.01.2018 | Erica Brunelle; Crystal Huynh; Eden Alin; Morgan Eldridge; Anh Minh Le; Lenka Halámková; Jan Halámek, Analytical Chemistry, 2018

Analytical Chemistry DOI: 10.1021/acs.analchem.8b00039

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Triple Signal Amplification Strategy for Ultrasensitive Determination of miRNA Based on Duplex Specific Nuclease and Bridge DNA–Gold Nanoparticles

18.01.2018 | Bing Bo; Tian Zhang; Yiting Jiang; Haiyan Cui; Peng Miao, Analytical Chemistry, 2018

Abnormal expression of miRNAs always occurs in solid tumors. Thus, it is critical to sensitively and selectively detect such biomarkers for the diagnosis and prognosis of diseases. Here, we report a biosensing scheme for the determination of miRNA with triple signal amplification based on ...

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