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10.410 Aktuelle Fachpublikationen in Analytical Chemistry

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3D Network and 2D Paper of Reduced Graphene Oxide/Cu2O Composite for Electrochemical Sensing of Hydrogen Peroxide

19.01.2018 | Chunfeng Cheng; Chunmei Zhang; Xiaohui Gao; Zhihua Zhuang; Cheng Du; Wei Chen, Analytical Chemistry, 2018

In this study, two-dimensional (2D) and three-dimensional (3D) freestanding reduced graphene oxide-supported Cu2O composites (Cu2O-rGO) were synthesized via simple and cost-efficient hydrothermal and filtration strategies. The structural characterizations clearly showed that highly porous 3D ...

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High Initial Sputter Rate Found for Vaccinia Virions Using Isotopic Labeling, NanoSIMS, and AFM

19.01.2018 | Sean D. Gates; Richard C. Condit; Nissin Moussatche; Benjamin J. Stewart; Alexander J. Malkin; Peter K. Weber, Analytical Chemistry, 2018

High-lateral-resolution secondary ion mass spectrometry (SIMS) has the potential to provide functional and depth resolved information from small biological structures, such as viral particles (virions) and phage, but sputter rate and sensitivity are not characterized at shallow depths relevant to ...

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Improving Mass Spectral Quality of Monoclonal Antibody Middle-Up LC-MS Analysis by Shifting the Protein Charge State Distribution

19.01.2018 | Wei Ding; Difei Qiu; Mark S. Bolgar; Scott A. Miller, Analytical Chemistry, 2018

Monoclonal antibodies (mAbs) are experiencing accelerated development in the pharmaceutical industry. Utilization of middle-up LC-MS methodology can provide detailed characterization of mAbs via reduction and/or enzymatic cleavage of the mAb into smaller protein fragments. However, under typical ...

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Highly Accurate Quantitative Analysis Of Enantiomeric Mixtures from Spatially Frequency Encoded 1H NMR Spectra

19.01.2018 | Bertrand Plainchont; Daisy Pitoux; Mathieu Cyrille; Nicolas Giraud, Analytical Chemistry, 2018

We propose an original concept to measure accurately enantiomeric excesses on proton NMR spectra, which combines high-resolution techniques based on a spatial encoding of the sample, with the use of optically active weakly orienting solvents. We show that it is possible to simulate accurately ...

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Aptamer-Based Dual-Functional Probe for Rapid and Specific Counting and Imaging of MCF-7 Cells

19.01.2018 | Bin Yang; Beibei Chen; Man He; Xiao Yin; Chi Xu; Bin Hu, Analytical Chemistry, 2018

Development of multimodal detection technologies for accurate diagnosis of cancer at early stages is in great demand. In this work, we report a novel approach using an aptamer-based dual-functional probe for rapid, sensitive, and specific counting and visualization of MCF-7 cells by inductively ...

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Correction to Fingerprint Analysis: Moving Toward Multiattribute Determination via Individual Markers

18.01.2018 | Erica Brunelle; Crystal Huynh; Eden Alin; Morgan Eldridge; Anh Minh Le; Lenka Halámková; Jan Halámek, Analytical Chemistry, 2018

Analytical Chemistry DOI: 10.1021/acs.analchem.8b00039

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Triple Signal Amplification Strategy for Ultrasensitive Determination of miRNA Based on Duplex Specific Nuclease and Bridge DNA–Gold Nanoparticles

18.01.2018 | Bing Bo; Tian Zhang; Yiting Jiang; Haiyan Cui; Peng Miao, Analytical Chemistry, 2018

Abnormal expression of miRNAs always occurs in solid tumors. Thus, it is critical to sensitively and selectively detect such biomarkers for the diagnosis and prognosis of diseases. Here, we report a biosensing scheme for the determination of miRNA with triple signal amplification based on ...

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Prussian Blue Nanoparticles as a Catalytic Label in a Sandwich Nanozyme-Linked Immunosorbent Assay

18.01.2018 | Zdeněk Farka; Veronika Čunderlová; Veronika Horáčková; Matěj Pastucha; Zuzana Mikušová; Antonín Hlaváček; Petr Skládal, Analytical Chemistry, 2018

Enzyme immunoassays are widely used for detection of analytes within various samples. However, enzymes as labels suffer several disadvantages such as high production cost and limited stability. Catalytic nanoparticles (nanozymes) can be used as an alternative label in immunoassays overcoming the ...

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Enzyme-Initiated Quinone-Chitosan Conjugation Chemistry: Toward A General in Situ Strategy for High-Throughput Photoelectrochemical Enzymatic Bioanalysis

18.01.2018 | Guang-Li Wang; Fang Yuan; Tiantian Gu; Yuming Dong; Qian Wang; Wei-Wei Zhao, Analytical Chemistry, 2018

Herein we report a general and novel strategy for high-throughput photoelectrochemical (PEC) enzymatic bioanalysis on the basis of enzyme-initiated quinone-chitosan conjugation chemistry (QCCC). Specifically, the strategy was illustrated by using a model quinones-generating oxidase of tyrosinase ...

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Determining Double Bond Position in Lipids Using Online Ozonolysis Coupled to Liquid Chromatography and Ion Mobility-Mass Spectrometry

17.01.2018 | Rachel A. Harris; Jody C. May; Craig A. Stinson; Yu Xia; John A. McLean, Analytical Chemistry, 2018

The increasing focus on lipid metabolism has revealed a need for analytical techniques capable of structurally characterizing lipids with a high degree of specificity. Lipids can exist as any one of a large number of double bond positional isomers, which are indistinguishable by single-stage mass ...

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