05.04.2013 | Chris E. Freye; Nichole A. Crane; Teresa B. Kirchner; Michael J. Sepaniak, Analytical Chemistry, 2013

A method for hyphenating surface enhanced Raman scattering (SERS) and thin-layer chromatography (TLC) is presented that employs silver–polymer nanocomposites as an interface. Through the process of conformal blotting, analytes are transferred from TLC plates to nanocomposite films before ...

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05.04.2013 | Yong Qiao; Jincui An; Liyuan Ma, Analytical Chemistry, 2013

This paper describes the use of a single cell array based assay for genotoxicity study of nanomaterials using normal human fetal fibroblast cells obtained from two-dimensional (2D) monolayer cultures and three-dimensional (3D) microtissue. After being exposed to a suspension of nanomaterials, ...

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04.04.2013 | Guangzhong Ma; Junyu Zhou; Chunxiu Tian; Dechen Jiang; Danjun Fang; Hongyuan Chen, Analytical Chemistry, 2013

A luminol electrochemiluminescence assay was reported to analyze active cholesterol at the plasma membrane in single mammalian cells. The cellular membrane cholesterol was activated by the exposure of the cells to low ionic strength buffer or the inhibition of intracellular ...

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04.04.2013 | Yongxin Li; Qingqing Wu; Shoufeng Jiao; Chaodi Xu; Lun Wang, Analytical Chemistry, 2013

A single Pt nanowire electrode (SPNE) was fabricated through HF etching process from Pt disk nanoelectrode and an underpotential deposition (UPD) redox replacement technique. The electrochemical experiments showed that SPNE had steady-state electrochemical responses at redox species solution ...

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04.04.2013 | Jeppe T. Pedersen; Niels H. H. Heegaard, Analytical Chemistry, 2013

Analytical Chemistry DOI: 10.1021/ac400023c

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03.04.2013 | Woong Hee Lee; Thomas R. Slaney; Robert W. Hower; Robert T. Kennedy, Analytical Chemistry, 2013

Microfabricated fluidic systems have emerged as a powerful approach for chemical analysis. Relatively unexplored is the use of microfabrication to create sampling probes. We have developed a sampling probe microfabricated in Si by bulk micromachining and lithography. The probe is 70 μm wide ...

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03.04.2013 | Kosuke Ino; Kaoru Ono; Toshiharu Arai; Yasufumi Takahashi; Hitoshi Shiku; Tomokazu Matsue, Analytical Chemistry, 2013

In this study, we fabricated a probe consisting of a carbon nanoelectrode and an Ag/AgCl reference electrode for detecting the activity of cells in single droplets. HeLa cells were confined into a single droplet, and the alkaline phosphatase (ALP) activity of the cells was electrochemically ...

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03.04.2013 | Abdul Rahim Ferhan; Longhua Guo; Xiaodong Zhou; Peng Chen; Seungpyo Hong; Dong-Hwan Kim, Analytical Chemistry, 2013

We introduce a novel solid-phase colorimetric sensor facilely fabricated by loading unmodified gold nanoparticles into poly(oligo(ethylene glycol)methacrylate) (POEGMA) brushes grown on glass. Our work reports the first synergistic combination of metallic nanoparticles acting as a ...

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03.04.2013 | Yuan Mao; Santosh G. Valeja; Jason C. Rouse; Christopher L. Hendrickson; Alan G. Marshall, Analytical Chemistry, 2013

Top-down electron capture dissociation (ECD) Fourier transform ion cyclotron resonance (FTICR) mass spectrometry was performed for structural analysis of an intact monoclonal antibody (IgG1kappa (κ) isotype, ∼148 kDa). Simultaneous ECD for all charge states (42+ to 58+) generates more ...

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03.04.2013 | Yoonhee Lee; Sung Hong Kwon; Youngkyu Kim; Jong-Bong Lee; Joon Won Park, Analytical Chemistry, 2013

Single-stranded 50-mer, 100-mer, and 150-mer DNAs were immobilized on a surface, and force-based atomic force microscopy (AFM) was employed to examine their behavior. A complementary 20-mer probe DNA on an AFM tip was used for the measurements. High-resolution maps were generated, and ...

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