15.04.2013 | Bett JS; Ibrahim AFM; Garg AK; Kelly V; Pedrioli P; Rocha S; Hay RT, Biochemical Journal, 2013

HIF1A (hypoxia-inducible factor 1α) is the master regulator of the cellular response to hypoxia and is implicated in cancer progression. Whereas the regulation of HIF1A protein in response to oxygen is well characterized, less is known about the fate of HIF1A mRNA. In the present study, we ...

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15.04.2013 | Sundberg EJ, Biochemical Journal, 2013

Asthma is a debilitating chronic inflammatory disease of the airways that results in shortness of breath, wheezing and coughing, often triggered by inhalation of common allergens. The prevalence and severity of asthma has increased dramatically over the last several decades to the point ...

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15.04.2013 | Zhou C; Zhou Y; Wang J; Feng Y; Wang H; Xue J; Chen Y; Ye RD; Wang M-W, Biochemical Journal, 2013

Genetic variation plays a major role in drug response variability. CsA (cyclosporin A), a widely used immunosuppressive agent, is a specific antagonist for FPR1 (formyl peptide receptor 1), which is an important G-protein-coupled chemoattractant receptor in the innate immune system. In order ...

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15.04.2013 | Hindley C; Philpott A, Biochemical Journal, 2013

PSCs (pluripotent stem cells) possess two key properties that have made them the focus of global research efforts in regenerative medicine: they have unlimited expansion potential under conditions which favour their preservation as PSCs and they have the ability to generate all somatic cell ...

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15.04.2013 | Khan SA; Rossi AM; Riley AM; Potter BVL; Taylor CW, Biochemical Journal, 2013

IP3R (IP3 [inositol 1,4,5-trisphosphate] receptors) and ryanodine receptors are the most widely expressed intracellular Ca2+ channels and both are regulated by thiol reagents. In DT40 cells stably expressing single subtypes of mammalian IP3R, low concentrations of thimerosal (also known as ...

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05.04.2013 | Song L; Bowen C; Gelmann E, Biochemical Journal, 2013

NKX3.1 is a prostate tumor suppressor protein that binds to topoisomerase I and enhances its DNA cleavage activity. We show that the NKX3.1 homeodomain binds to a region of topoisomerase I spanning the junction between core and linker domains. NKX3.1 activated N-terminal truncated ...

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05.04.2013 | Gisler C; L{u}scher D; Sch{a}tzle P; D{u}rr S; Baici A; Galliciotti G; Reif R; Bolliger M; Kunz B; Sonderegger P, Biochemical Journal, 2013

The serine peptidase neurotrypsin is stored in presynaptic nerve endings and secreted in an inactive, zymogenic form by synaptic activity. After activation, which requires activity of postsynaptic NMDA receptors, neurotrypsin cleaves the heparan sulfate proteoglycan agrin at active synapses. ...

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05.04.2013 | Kim W; Hsiao J; Bhatia S; Glaros E; Don A; Tsuruoka S; Shannon Weickert C; Halliday G, Biochemical Journal, 2013

Members of ATP-binding cassette subfamily A (ABCA) are characterized by their ability to transport lipids across cellular membranes and regulate lipid homeostasis in the brain and peripheral tissues. ABCA8 is a little-known member of this subfamily, originally cloned from human brain ...

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05.04.2013 | Davies P; Hinkle K; Sukar N; Sepulveda B; Mesias R; Serrano G; Alessi D; Beach T; Benson D; White III C; Cowell R; D ..., Biochemical Journal, 2013

Missense mutations in leucine-rich repeat kinase 2 (LRRK2) are a major cause of Parkinson’s disease (PD). Several antibodies against LRRK2 have been developed, but results using these polyclonal antibodies have varied widely leading to conflicting conclusions. To address this challenge, The ...

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04.04.2013 | Foley K; Young P, Biochemical Journal, 2013

The non-muscle α-actinin isoforms (actinin-1 and -4) are closely related, dimeric actin filament-crosslinking proteins. Despite high sequence similarity, unique properties have been ascribed to actinin-4 in particular. For example, actinin-4, but not actinin-1, is essential for normal ...

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