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297 Aktuelle Fachpublikationen zum Thema Addition

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Concerted Protein and Nucleic Acid Conformational Changes Observed Prior to Nucleotide Incorporation in a Bacterial RNA Polymerase: Raman Crystallographic Evidence

18.08.2015 | Ioanna H. Antonopoulos; Brittany A. Warner; Paul R. Carey, Biochemistry, 2015

Transcription elongation requires the continuous incorporation of ribonucleotide triphosphates into a growing transcript. RNA polymerases (RNAPs) are able to processively synthesize a growing RNA chain via translocation of the RNAP enzyme along its nucleic acid template strand after each ...


Role of GxxxG Motifs in Transmembrane Domain Interactions

13.08.2015 | Mark G. Teese; Dieter Langosch, Biochemistry, 2015

Transmembrane (TM) helices of integral membrane proteins can facilitate strong and specific noncovalent protein–protein interactions. Mutagenesis and structural analyses have revealed numerous examples in which the interaction between TM helices of single-pass membrane proteins is dependent on a ...


Metal Stabilization of Collagen and de Novo Designed Mimetic Peptides

10.08.2015 | Avanish S. Parmar; Fei Xu; Douglas H. Pike; Sandeep V. Belure; Nida F. Hasan; Kathryn E. Drzewiecki; David I. Shreib ..., Biochemistry, 2015

We explore the design of metal binding sites to modulate triple-helix stability of collagen and collagen-mimetic peptides. Globular proteins commonly utilize metals to connect tertiary structural elements that are well separated in sequence, constraining structure and enhancing stability. It is ...


Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range

04.08.2015 | Claudia Solscheid; Simone Kunzelmann; Colin T. Davis; Jackie L. Hunter; Annie Nofer; Martin R. Webb, Biochemistry, 2015

A fluorescent reagentless biosensor for inorganic phosphate (Pi), based on the E. coli PstS phosphate binding protein, was redesigned to allow measurements of higher Pi concentrations and at low, substoichiometric concentrations of biosensor. This was achieved by weakening Pi binding of the ...


Importance of β2–β3 Loop Motion for the Increased Binding and Decreased Selectivity of the ΔLL Mutant of the Human Papillomavirus Type 6 E2 Protein

30.07.2015 | Geoffrey M. Gray; Arjan van der Vaart, Biochemistry, 2015

The binding affinity of the human papillomavirus type 6 E2 protein is strongly mediated by the sequence of the DNA linker region, with high affinity for the AATT linker and low affinity for the CCGG linker. When two terminal leucine residues are removed from the protein, the level of binding to ...


Systematic Perturbations of Binuclear Non-heme Iron Sites: Structure and Dioxygen Reactivity of de Novo Due Ferri Proteins

24.07.2015 | Rae Ana Snyder; Justine Betzu; Susan E. Butch; Amanda J. Reig; William F. DeGrado; Edward I. Solomon, Biochemistry, 2015

DFsc (single-chain due ferri) proteins allow for modeling binuclear non-heme iron enzymes with a similar fold. Three 4A → 4G variants of DFsc were studied to investigate the effects of (1) increasing the size of the substrate/solvent access channel (G4DFsc), (2) including an additional His ...


Decoding the Matrix: Instructive Roles of Proteoglycan Receptors

22.07.2015 | Thomas Neill; Liliana Schaefer; Renato V. Iozzo, Biochemistry, 2015

The extracellular matrix is a dynamic repository harboring instructive cues that embody substantial regulatory dominance over many evolutionarily conserved intracellular activities, including proliferation, apoptosis, migration, motility, and autophagy. The matrix also coordinates and parses ...


Neural Zinc Finger Factor/Myelin Transcription Factor Proteins: Metal Binding, Fold, and Function

16.07.2015 | Angelique N. Besold; Sarah L. J. Michel, Biochemistry, 2015

Zinc finger (ZF) proteins make up a large family of metalloproteins that contain discrete domains with amino acid ligands (cysteine and histidine) that serve to coordinate zinc in a tetrahedral geometry. Upon zinc coordination, the domains adopt three-dimensional structure. The most well-studied ...


Chemically Induced Changes to Membrane Permeability in Living Cells Probed with Nonlinear Light Scattering

15.07.2015 | Michael J. Wilhelm; Mohammad Sharifian Gh.; Hai-Lung Dai, Biochemistry, 2015

Second-harmonic light scattering (SHS) permits characterization of membrane-specific molecular transport in living cells. Herein, we demonstrate the use of time-resolved SHS for quantifying chemically induced enhancements in membrane permeability. As proof of concept, we examine the enhanced ...


G Protein-Coupled Receptor Kinase 2 (GRK2) and 5 (GRK5) Exhibit Selective Phosphorylation of the Neurotensin Receptor in Vitro

08.07.2015 | Sayaka Inagaki; Rodolfo Ghirlando; Sergey A. Vishnivetskiy; Kristoff T. Homan; Jim F. White; John J. G. Tesmer; Vsev ..., Biochemistry, 2015

G protein-coupled receptor kinases (GRKs) play an important role in the desensitization of G protein-mediated signaling of G protein-coupled receptors (GPCRs). The level of interest in mapping their phosphorylation sites has increased because recent studies suggest that the differential pattern ...


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