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Integrated strategy for high-sensitive phosphoproteome analysis from low micrograms of protein samples

20.06.2018 | Wendong Chen; Lan Chen; Ruijun Tian, Analyst, 2018

Phosphoproteomics has become popular proteomic technology for exploring cellular signaling networks. However, current approaches often require milligrams of protein samples which hamper their applications for translational purpose with limited starting materials. In this study, we aimed to ...

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Heteroleptic Cu(I) complexes bearing methoxycarbonyl-imidoylindazole and POP ligands - An experimental and theoretical study of their photophysical properties

20.06.2018 | Iván Alonso González-Pavez; Marco Antonio Henriquez; Diego Cortés-Arriagada; Mirco Natali; Constantin ..., New Journal of Chemistry, 2018

Four new mixed ligand Cu(I) complexes bearing methoxycarbonyl imidoyl-indazole and bis[2-(diphenylphosphino)-phenyl]ether (POP) ligands were synthesized and characterized by variable-temperature NMR, FT-IR, EA and HRMS. For three of them, the molecular structures were obtained by X-ray ...

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Interactions between H-bonded [CuII3([small mu ]3-OH)] triangles; a combined magnetic susceptibility and EPR study

20.06.2018 | Logesh Mathivathanan; Athanassios K. Boudalis; Philippe Turek; Michael Pissas; Yiannis Sanakis; Raphael G. Raptis, Physical Chemistry Chemical Physics, 2018

The X-ray crystal structure of the CuII complex [Cu3(μ3-OH)(μ-pz)3(PhCOO)3]− (pz− = pyrazolato anion) shows an isosceles triangular core, further forming a hexanuclear H-bonded aggregate. Cleavage of the H-bonds in solution results in isolated trinuclear species. Analysis of variable temperature ...

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Multi-Functional Derivatization of Amine, Hydroxyl, and Carboxylate Groups for Metabolomic Investigations of Human Tissue by Electrospray Ionization Mass Spectrometry

20.06.2018 | Tianjiao Huang; Maria Toro-Moreno; Dawn S Hui; Richard Lee; James L Edwards, Analyst, 2018

Metabolomics, the study of small molecules involved in cellular processes, offers the potential to reveal insights into the pathophysiology of disease states. Analysis of metabolites by electrospray mass spectrometry is complicated by their structural diversity. Amine, hydroxyl, and carboxylate ...

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Sequential evaporation of water molecules from protonated water clusters: measurement of the velocity distributions of the evaporated molecules and statistical analysis

20.06.2018 | Francis Berthias; Linda Feketeova; Hassan Abdoul-Carime; Florent Calvo; Bernadette Farizon; Michel Farizon; Tilmann ..., Physical Chemistry Chemical Physics, 2018

Velocity distributions of water molecules evaporated from protonated water clusters H+(H2O)n≤ 10 after collision-induced dissociation (CID) were measured using the combined correlated ion and neutral fragment time-of-flight (COINTOF) and velocity map imaging (VMI) techniques. As observed ...

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Occurrence and source analysis of selected antidepressants and their metabolites in municipal wastewater and receiving surface water

20.06.2018 | Luo-dan Ma; Jian Li; Jia-jun Li; Min Liu; Dong-zhi Yan; Wen-yan Shi; Gang Xu, Journal of Environmental Monitoring, 2018

Seven commonly utilized antidepressants (amitriptyline, fluoxetine, sertraline, citalopram, paroxetine, venlafaxine, and bupropion) and three of their metabolites were detected in four wastewater treatment plants (WWTPs) and corresponding receiving waters including the mainstream and three of the ...

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General and stereoselective aminoxylation of biradical titanium(IV) enolates with TEMPO: a detailed study on the effect of the chiral auxiliary

20.06.2018 | Pedro Romea; Fèlix Urpí; Stuart Christopher David Kennington; Alejandro Gómez-Palomino; Ernest Salom& ..., Organic & Biomolecular Chemistry, 2018

A comprehensive analysis of the influence of the chiral auxiliary on the α-aminoxylation of titanium(IV) enolates with TEMPO indicated that (S) 4-tert-butyl-1-oxazolidine-2-thione is the most appropriate scaffold to provide a single diastereomer in high yields for a variety of substrates, which ...

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Internal standard fused glass beads for high silicon content sample analysis by laser-induced breakdown spectrometry

20.06.2018 | Alexandrina A. C. Carvalho; Flavio O. Leme; Maciel S. Luz; Pedro V. Oliveira; Cassiana S. Nomura, Journal of Analytical Atomic Spectrometry, 2018

The direct analysis of high silicon content samples by Laser-Induced Breakdown Spectrometry (LIBS) is a good alternative, since the decomposition of samples with refractory characteristics is not an easy task. However, poor precision and difficulty in performing quantitative analysis due to ...

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Static droplet array for culturing single live adherent cells in an isolated chemical microenvironment

20.06.2018 | Amin Hassanzadeh-Barforoushi; Andrew Law; Abbas Hejri; Mohsen Asadnia; David Gallego-Ortega; Christopher Ormandy; ..., Lab on a Chip, 2018

We present here a new method to easily and reliably generate an array of hundreds of dispersed nanoliter-volume semi-droplets for single-cells culture and analysis. The liquid segmentation step occurs directly in indexed traps by a tweezer-like mechanism and is stabilized by spatial confinement. ...

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Life cycle assessment and techno-economic analysis of the utilization of bio-oil components for the production of three chemicals

20.06.2018 | JiLu zheng; Ya-Hong Zhu; Ming-Qiang Zhu; Guotao Sun; Run-Cang Sun, Green Chemistry, 2018

Bio-based chemicals have gained more widespread attention than bio-fuels because of the decline in crude oil prices. The development of technologies to produce chemicals from bio-oil is potentially attractive because of the simplicity of bio-oil preparation. This study presents a prospective and ...

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