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103.960 Aktuelle Fachpublikationen zum Thema temperature

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Dual-view plane illumination microscopy for rapid and spatially isotropic imaging

01.11.2014 | Abhishek Kumar; Yicong Wu; Ryan Christensen; Panagiotis Chandris; William Gandler; Evan McCreedy; Alexandra Bokinsky ..., Nature Protocols, 2014

Nature Protocols 9, 2555 (2014). doi:10.1038/nprot.2014.172 Authors: Abhishek Kumar, Yicong Wu, Ryan Christensen, Panagiotis Chandris, William Gandler, Evan McCreedy, Alexandra Bokinsky, Daniel A Colón-Ramos, Zhirong Bao, Matthew McAuliffe, Gary Rondeau & Hari Shroff We ...


Influenza A virus isolation, culture and identification

01.11.2014 | Amie J Eisfeld; Gabriele Neumann; Yoshihiro Kawaoka, Nature Protocols, 2014

Nature Protocols 9, 2663 (2014). doi:10.1038/nprot.2014.180 Authors: Amie J Eisfeld, Gabriele Neumann & Yoshihiro Kawaoka Influenza A viruses (IAVs) cause epidemics and pandemics that result in considerable financial burden and loss of human life. To manage annual IAV epidemics ...


Sensitive impedimetric biosensor based on duplex–like DNA scaffolds and ordered mesoporous carbon nitride for silver(I) ion detection

22.10.2014 | Yaoyu Zhou; Lin Tang; Xia Xie; Guangming Zeng; Jiajia Wang; Yaocheng Deng; Guide Yang; Chen Zhang; Yi Zhang; Jun Chen, Analyst, 2014

This study demonstrates a new unlabeled immobilized DNA–based biosensor with ordered mesoporous carbon nitride material (MCN) for detection of Ag+ by electrochemical impedance spectroscopy (EIS) with [Fe(CN)6]4–/3– as redox couple. The unlabeled immobilized DNA initially formed the hairpin ...


Sensitive marker bands for the detection of spin states of heme in surface-enhanced resonance Raman scattering spectra of metmyoglobin

22.10.2014 | Yasutaka Kitahama; Masatoshi Egashira; Toshiaki Suzuki; Ichiro Tanabe; Yukihiro Ozaki, Analyst, 2014

Surface-enhanced resonance Raman scattering (SERRS) spectra of myoglobin (Mb) with various ligands were measured. In the resonance Raman scattering (RRS) spectra, the peaks around at 1610 and 1640 cm–1 have been used to discriminate between the heme iron in high or low spin state so far. In the ...


Propofol detection and quantification in human blood: the promise of feedback controlled, closed-loop anesthesia

22.10.2014 | Francine Kivlehan; Edward Chaum; Ernő Lindner, Analyst, 2014

The performance of a membrane-coated voltammetric sensor for propofol (2,6-diisopropylphenol) has been characterized in long term monitoring experiments using an automated flow analytical system (AFAS) and by analyzing human serum and whole blood samples by standard addition. It is shown that the ...


Microscale Anechoic Architecture: Acoustic Diffusers for Ultra Low Power Microparticle Separation via Traveling Surface Acoustic Waves

22.10.2014 | Jan Behrens; Sean Langelier; Amgad Rezk; Gerhard Lindner; Leslie Yeo; James R. Friend, Lab on a Chip, 2014

We present a versatile and very low-power traveling SAW microfluidic sorting device able to displace and separate particles of different diameter in aqueous suspension; the travelling wave propagates through the fluid bulk and diffuses via a Schröder diffuser, adopted from its typical use in ...


Microfluidic pipette array for mechanophentyping of cancer cells and mechanical gating of mechanosensitive channels

22.10.2014 | Lap Man Lee; Allen Liu, Lab on a Chip, 2014

Micropipette aspiration measures the mechanical properties of single cells. A traditional micropipette aspiration system requires a bulky infrastructure, and has a low throughput and limited potential for automation. We have developed a simple microfluidic device, which is able to trap and apply ...


Plasmonic aptamer–gold nanoparticle sensors for small molecule fingerprint identification

22.10.2014 | Jorge L. Chávez; Juliann K. Leny; Suzanne Witt; Grant M. Slusher; Joshua A. Hagen; Nancy Kelley-Loughnane, Analyst, 2014

The utilization of the plasmonic response of aptamer–gold nanoparticle conjugates (Apt–AuNPs) to design cross-reactive arrays for fingerprint identification of small molecular targets was demonstrated for the first time. Four aptamers with different structural features previously selected to bind ...


In vitro HER2 protein-induced affinity dissociation of carbon nanotube-wrapped anti-HER2 aptamers for HER2 protein detection

22.10.2014 | Javed H. Niazi; Sandeep Kumar Verma; Sarfaraj Niazi; Anjum Qureshi, Analyst, 2014

A new in vitro assay was developed to detect human epidermal growth factor receptor 2 (HER2) protein based on affinity dissociation of carbon nanotube (CNT)-wrapped anit-HER2 ssDNA aptamers. First, we selected an anti-HER2 ssDNA aptamer (H2) using an in vitro Serial Evolution of Ligands by ...


Hydrazinonicotinic acid as a novel matrix for highly sensitive and selective MALDI-MS analysis of oligosaccharides

21.10.2014 | Jing Jiao; Ying Zhang; Pengyuan Yang; Haojie Lu, Analyst, 2014

Analysis of oligosaccharides with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) remains challenging due to their low ionization efficiency. The sensitivity achieved by MS for oligosaccharides lags far behind that for proteins/peptides. Here, the hydrazinonicotinic acid ...


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