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36 Aktuelle Fachpublikationen zum Thema temperature

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In vitro dihydrouridine formation by tRNA dihydrouridine synthase from Thermus thermophilus, an extreme-thermophilic eubacterium

01.12.2015 | Hiroaki Kusuba; Takeshi Yoshida; Eri Iwasaki; Takako Awai; Ai Kazayama; Akira Hirata; Chie Tomikawa; Ryota Yamagami; ..., Journal of Biochemistry, 2015

Dihydrouridine (D) is formed by tRNA dihydrouridine synthases (Dus). In mesophiles, multiple Dus enzymes bring about D modifications at several positions in tRNA. The extreme-thermophilic eubacterium Thermus thermophilus, in contrast, has only one dus gene in its genome and only two D ...

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Molecular and biochemical characterization of bifunctional pyruvate decarboxylases and pyruvate ferredoxin oxidoreductases from Thermotoga maritima and Thermotoga hypogea

01.12.2015 | Mohammad S. Eram; Alton Wong; Erica Oduaran; Kesen Ma, Journal of Biochemistry, 2015

Hyperthermophilic bacteria Thermotoga maritima and Thermotoga hypogea produce ethanol as a metabolic end product, which is resulted from acetaldehyde reduction catalysed by an alcohol dehydrogenase (ADH). However, the enzyme that is involved in the production of acetaldehyde from pyruvate is not ...

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Crack tip shielding observed with high-resolution transmission electron microscopy

01.10.2015 | Damar Rastri Adhika; Masaki Tanaka; Takeshi Daio; Kenji Higashida, Journal of Electron Microscopy, 2015

The dislocation shielding field at a crack tip was experimentally proven at the atomic scale by measuring the local strain in front of the crack tip using high-resolution transmission electron microscopy (HRTEM) and geometric phase analysis (GPA). Single crystalline (110) silicon wafers were ...

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Temperature-sensitive retinoid isomerase activity of RPE65 mutants associated with Leber Congenital Amaurosis

01.08.2015 | Songhua Li; Jane Hu; Robin J. Jin; Ashok Aiyar; Samuel G. Jacobson; Dean Bok; Minghao Jin, Journal of Biochemistry, 2015

RPE65 is a membrane-associated retinoid isomerase involved in the visual cycle responsible for sustaining vision. Many mutations in the human RPE65 gene are associated with distinct forms of retinal degenerative diseases. The pathogenic mechanisms for most of these mutations remain poorly ...

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Temperature Affects Human Sweet Taste via At Least Two Mechanisms

01.07.2015 | Barry G. Green; Danielle Nachtigal, Chemical Senses, 2015

The reported effects of temperature on sweet taste in humans have generally been small and inconsistent. Here, we describe 3 experiments that follow up a recent finding that cooling from 37 to 21 °C does not reduce the initial sweetness of sucrose but increases sweet taste adaptation. In ...

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Thermodynamic analysis of unusually thermostable CutA1 protein from human brain and its protease susceptibility

01.03.2015 | Bagautdin Bagautdinov; Yoshinori Matsuura; Hitoshi Yamamoto; Masahide Sawano; Kyoko Ogasahara; Michiyo Takehira; Nao ..., Journal of Biochemistry, 2015

Unusually stable proteins are a disadvantage for the metabolic turnover of proteins in cells. The CutA1 proteins from Pyrococcus horikoshii and from Oryza sativa (OsCutA1) have unusually high denaturation temperatures (Td) of nearly 150 and 100°C, respectively, at pH 7.0. It seemed that the CutA1 ...

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In situ transmission electron microscopy observation of dislocation motion in 9Cr steel at elevated temperatures: influence of shear stress on dislocation behavior

01.12.2014 | Susumu Yamada; Takayuki Sakai, Journal of Electron Microscopy, 2014

To elucidate high-temperature plastic deformation (creep) mechanism in materials, it is essential to observe dislocation motion under tensile loading. There are many reports on in situ transmission electron microscopy (TEM) observations in the literature; however, the relationship between the ...

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Atomic force microscope-assisted scanning tunneling spectroscopy under ambient conditions

01.12.2014 | Amin Vakhshouri; Katsushi Hashimoto; Yoshiro Hirayama, Journal of Electron Microscopy, 2014

We have developed a method of atomic force microscopy (AFM)-assisted scanning tunneling spectroscopy (STS) under ambient conditions. An AFM function is used for rapid access to a selected position prior to performing STS. The AFM feedback is further used to suppress vertical thermal drift of the ...

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Conformational stability, reversibility and heat-induced aggregation of {alpha}-1-acid glycoprotein

01.12.2014 | Takafumi Iwura; Jun Fukuda; Katsuyoshi Yamazaki; Fumio Arisaka, Journal of Biochemistry, 2014

To investigate the relationship between conformational stability, reversibility of denaturation and aggregation of protein, we determined the conformation, melting temperature (Tm), and reversibility of heat-induced denaturation of α-1-acid glycoprotein (AGP) in aqueous solutions at various pH ...

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The Effect of Temperature and Menthol on Carbonation Bite

01.09.2014 | Paul M. Wise; Bruce Bryant, Chemical Senses, 2014

Temperature and chemesthesis interact, but this interaction has not been fully examined for most irritants. The current experiments focus on oral pungency from carbonation. Previous work showed that cooling carbon dioxide (CO2) solutions to below tongue temperature enhanced rated bite. However, ...

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