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31 Aktuelle Fachpublikationen zum Thema temperature

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Thermodynamic analysis of unusually thermostable CutA1 protein from human brain and its protease susceptibility

01.03.2015 | Bagautdin Bagautdinov; Yoshinori Matsuura; Hitoshi Yamamoto; Masahide Sawano; Kyoko Ogasahara; Michiyo Takehira; Nao ..., Journal of Biochemistry, 2015

Unusually stable proteins are a disadvantage for the metabolic turnover of proteins in cells. The CutA1 proteins from Pyrococcus horikoshii and from Oryza sativa (OsCutA1) have unusually high denaturation temperatures (Td) of nearly 150 and 100°C, respectively, at pH 7.0. It seemed that the CutA1 ...


In situ transmission electron microscopy observation of dislocation motion in 9Cr steel at elevated temperatures: influence of shear stress on dislocation behavior

01.12.2014 | Susumu Yamada; Takayuki Sakai, Journal of Electron Microscopy, 2014

To elucidate high-temperature plastic deformation (creep) mechanism in materials, it is essential to observe dislocation motion under tensile loading. There are many reports on in situ transmission electron microscopy (TEM) observations in the literature; however, the relationship between the ...


Atomic force microscope-assisted scanning tunneling spectroscopy under ambient conditions

01.12.2014 | Amin Vakhshouri; Katsushi Hashimoto; Yoshiro Hirayama, Journal of Electron Microscopy, 2014

We have developed a method of atomic force microscopy (AFM)-assisted scanning tunneling spectroscopy (STS) under ambient conditions. An AFM function is used for rapid access to a selected position prior to performing STS. The AFM feedback is further used to suppress vertical thermal drift of the ...


Conformational stability, reversibility and heat-induced aggregation of {alpha}-1-acid glycoprotein

01.12.2014 | Takafumi Iwura; Jun Fukuda; Katsuyoshi Yamazaki; Fumio Arisaka, Journal of Biochemistry, 2014

To investigate the relationship between conformational stability, reversibility of denaturation and aggregation of protein, we determined the conformation, melting temperature (Tm), and reversibility of heat-induced denaturation of α-1-acid glycoprotein (AGP) in aqueous solutions at various pH ...


The Effect of Temperature and Menthol on Carbonation Bite

01.09.2014 | Paul M. Wise; Bruce Bryant, Chemical Senses, 2014

Temperature and chemesthesis interact, but this interaction has not been fully examined for most irritants. The current experiments focus on oral pungency from carbonation. Previous work showed that cooling carbon dioxide (CO2) solutions to below tongue temperature enhanced rated bite. However, ...


Gustatory Receptor Neurons in Manduca sexta Contain a TrpA1-Dependent Signaling Pathway that Integrates Taste and Temperature

01.09.2013 | Anika Afroz; Natalie Howlett; Aditi Shukla; Farah Ahmad; Elizabeth Batista; Katie Bedard; Sara Payne; Brian Morton; ..., Chemical Senses, 2013

Temperature modulates the peripheral taste response of many animals, in part by activating transient receptor potential (Trp) cation channels. We hypothesized that temperature would also modulate peripheral taste responses in larval Manduca sexta. We recorded excitatory responses of the lateral ...


Renewed interest in basic and applied research involving monoclonal antibodies against an oncofetal Tn-antigen

01.08.2013 | Yoko Fujita-Yamaguchi, Journal of Biochemistry, 2013

Tn-antigen (GalNAcα-Ser/Thr) is one of the most common aberrations associated with cancer progression and metastasis, and thus is an excellent target for development of cancer diagnostics and therapeutics. MLS128 monoclonal antibody (mAb), derived from a mouse immunized with human colon carcinoma ...


Sample heating system for spin-polarized scanning electron microscopy

01.08.2013 | Teruo Kohashi; Kumi Motai, Journal of Electron Microscopy, 2013

A sample-heating system for spin-polarized scanning electron microscopy (spin SEM) has been developed and used for microscopic magnetization analysis at temperatures up to 500°C. In this system, a compact ceramic heater and a preheating operation keep the ultra-high vacuum conditions while the ...


Biochemical characterization of highly active Trypanosoma brucei gambiense glycerol kinase, a promising drug target

01.07.2013 | Emmanuel Oluwadare Balogun; Daniel Ken Inaoka; Tomoo Shiba; Yasutoshi Kido; Takeshi Nara; Takashi Aoki; Teruki Honma ..., Journal of Biochemistry, 2013

Human African trypanosomes are blood parasites that cause sleeping sickness, a debilitating disease in sub-Saharan Africa. Glycerol kinase (GK) of these parasites additionally possesses a novel property of reverse catalysis. GK is essential to blood stream form trypanosome, and therefore a ...


Interaction of wheat {beta}-amylase with maltose and glucose as examined by fluorescence

01.07.2013 | Tadessa Daba; Kenji Kojima; Kuniyo Inouye, Journal of Biochemistry, 2013

Fluorescence of wheat β-amylase (WBA) was quenched by the interaction with maltose or glucose, which are competitive inhibitors of WBA, suggesting that the states of tryptophan and tyrosine residues could be changed by the interaction. The fluorescence emitted by excitation at 280 and 295 nm was ...


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