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9 Aktuelle Fachpublikationen zum Thema temperature

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Fever-like temperature modification differentially affects in vitro signaling of bradykinin B1 and B2 receptors

28.08.2011 | Jasmin Leschner; Larisa Ring; Jens Feierler; Klaus Dinkel; Marianne Jochum; Alexander Faussner, Biological Chemistry, 2011

Abstract The bradykinin (BK) B2 and B1 receptors (B2R, B1R) belong to the rhodopsin-like G protein-coupled receptors (GPCRs) and are involved in (patho)physiological processes such as blood pressure regulation or inflammation. They mediate the effects of the pro-inflammatory peptides ...

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Effect of sample collection, temperature and time of storage on β-galactosidase and total hexosaminidase activities in dried blood collected on filter paper

17.05.2011 | Cristina D. de Castilhos; Jamila Mezzalira; Mariana P.S. Goldim; Frederico G. Werlang; Janice C. Coelho, Clinical Chemistry , 2011

Abstract Background: Dried blood spots (DBS) on filter paper is a valuable sampling technique in clinical chemistry, but the stability of enzymes used in the diagnosis of lysosomal storage diseases (LSDs) needs to be evaluated. Methods: In a first experiment, blood from 20 subjects was collected ...

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Monoclonal gammopathy missed by capillary zone electrophoresis

17.05.2011 | Christof Schild; Florence Egger; Alain Kaelin-Lang; Jean-Marc Nuoffer, Clinical Chemistry , 2011

Abstract Background: Serum protein electrophoresis is used as a screening test for monoclonal gammopathies. Here, we present a case of a high-concentration monoclonal immunoglobulin (M-protein) that was missed by serum protein electrophoresis on a Capillarys 2 capillary zone electrophoresis ...

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Adaptation of the Diazyme Direct Enzymatic HbA1c Assay for a microplate reader at room temperature

17.05.2011 | Ilkka Penttilä; Karri Penttilä; Toivo Halonen; Kari Pulkki; Jukka Törrönen; Rainer Rauramaa, Clinical Chemistry , 2011

Abstract Background: Measurement of hemoglobin A1c (HbA1c) is a key diagnostic criterion and a key parameter for the follow-up of the treatment of diabetes mellitus. Typically, immunochemical assays of HbA1c are performed in clinical chemistry analyzers. In this study, we applied the HbA1c assay ...

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Rapid microwave digestion and microplate reading format method for urinary iodine determination

22.02.2011 | Mehdi Hedayati; Marjan Khazan; Parichehr Yaghmaee; Marjan Zarif Yeghaneh; Leila Behdadfar; Maryam Sadat Daneshpour, Clinical Chemistry , 2011

Abstract Background: High temperature and long heating time can be considered the main drawbacks of the conventional digestion methods of urinary iodine determination. The aim of this study was to assess the usefulness of a microwave for digestion and decomposition of urine interfering ...

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Impact of cryopreservation on serum concentration of matrix metalloproteinases (MMP)-7, TIMP-1, vascular growth factors (VEGF) and VEGF-R2 in Biobank samples

22.02.2011 | Kalle Kisand; Irina Kerna; Jaanika Kumm; Helgi Jonsson; Agu Tamm, Clinical Chemistry , 2011

Abstract Background: Blood biomarkers are subject to pre-analytical variability. In many cases, the stability of important new tissue biomarkers during freeze cycles and storage has not been studied sufficiently. Methods: To test the stability of matrix metalloproteinases-7 (MMP-7) and their ...

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Physiology and pathophysiology of the RANKL/RANK system

18.11.2010 | Reiko Hanada; Toshikatsu Hanada; Josef M. Penninger, Biological Chemistry, 2010

Abstract The TNF family molecule RANKL and its receptor RANK are key regulators of bone remodeling, lymph node formation, and mammary gland development during pregnancy. RANKL and RANK are also expressed in the central nervous systems (CNS). However, the functional relevance of RANKL/RANK in the ...

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Effect of storage temperature or collection tube type on blood methylmalonic acid concentrations measured by gas chromatography-mass spectrometry

21.09.2010 | Edmunds Z. Reineks; Anita Hughes; Sihe Wang, Clinical Chemistry , 2010

Clinical Chemistry and Laboratory Medicine, Volume 48, Issue 9, Page 1351-1353, September 2010.

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Small-angle X-ray and neutron scattering as a tool for structural systems biology

12.07.2010 | Dmitri I. Svergun, Biological Chemistry, 2010

Abstract Small-angle scattering (SAS) of X-rays and neutrons reveals low-resolution structures of biological macromolecules in solution. With the recent experimental and methodological advances, SAS became a unique tool for characterising biological systems. The method covers an extremely broad ...

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