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Robust fluorescent labelling of micropipettes for use in fluorescence microscopy: application to the observation of a mosquito borne parasite infection

Summary

The ability to monitor micropipette injections with a high‐resolution fluorescent microscope has utility for a variety of applications. Herein, different approaches were tested for creating broad‐band fluorescently labelled glass micropipettes including: UV cured glass glues, baked glass enamel containing fluorescent dyes as well as nanodiamonds attached during pipette formation in the microforge. The most robust and simplest approach was to use labelled baked enamel on the exterior of the pipette. This approach was tested using pipettes designed to mimic a mosquito proboscis for the injection of the malaria parasite, Plasmodium spp., into the dermis of a living mouse ear. The pipette (∼30 micron diameter) was easily detected in the microscopy field of view and tolerated multiple insertions through the skin. This simple inexpensive approach to fluorescently labelling micropipettes will aid in the development of procedures under the fluorescent microscope.

Lay description

A technique is described to fluorescently label glass micropipettes. Fluorescent pipettes permit the direct monitoring of injection of agents under a high‐resolution fluorescent microscope. Using a fluorescent micropipette system that mimics the mosquito bite permitted the observation of the initial skin invasion of malaria parasites in the living mouse ear.

Autoren:   AMANDA E. BALABAN, KEIR NEUMAN, PHOTINI SINNIS, ROBERT S. BALABAN
Journal:   Journal of Microscopy
Jahrgang:   2017
Seiten:   n/a
DOI:   10.1111/jmi.12610
Erscheinungsdatum:   10.08.2017
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