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Epitope‐Resolved Detection of Peanut‐Specific IgE Antibodies by Surface Plasmon Resonance Imaging

The front cover picture shows the selective binding of IgE‐coated magnetic beads onto allergen‐functionalized array spots. Binding is detected by surface plasmon resonance imaging (SPRi), as depicted with the reflected red light below the array. Anti‐IgE antibodies and peptide and carbohydrate antigens from the peanut protein Ara h2 were selectively attached to individual array spots; this is shown by the different features on the gold dots. The beads were prepared by selective capture of IgEs from serum by IgE ϵ‐chain‐specific antibody‐coated particles, and differentiated in a microfluidic flow system. Only spots on the array containing specific allergenic epitopes bound the beads, and binding correlated with a clinical measure of peanut allergy. More information can be found in the communication by M. W. Peczuh et al.

Autoren:   Min Shen, Amit A. Joshi, Raghu Vannam, Chandra K. Dixit, Robert G. Hamilton, Challa V. Kumar, James F. Rusling, Mark W. Peczuh
Journal:   ChemBioChem
Jahrgang:   2018
Seiten:   n/a
DOI:   10.1002/cbic.201700694
Erscheinungsdatum:   15.01.2018
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