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Investigating the accessibility of nucleosome by duocarmycin B2, Fe(II) peplomycin and MNase using capillary electrophoresis

Capillary electrophoresis coupled with DNA 5 Texas Red labeling was used to investigate the ability of duocarmycin B2, Fe(II) peplomycin, and MNase to access the nucleosome. Distinct accessibility patterns of these species to the nucleosome were observed. Duocarmycin B2 was able to enter and react at the core region, although its alkylating efficiency decreased significantly. Intercalation of peplomycin in the nucleosomal core region was highly suppressed, but reaction sites located at the ends of the nucleosomal core remained accessible, which implied the flexibility of the core DNA end. MNase was completely prevented from approaching the nucleosome core and exhibited a higher site specificity for targeting DNA sites located close to the core region.

Autoren:   Tingting Zou, Seiichiro Kizaki, Hiroshi Sugiyama
Journal:   ChemBioChem
Jahrgang:   2018
Seiten:   n/a
DOI:   10.1002/cbic.201700643
Erscheinungsdatum:   15.01.2018
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