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Identification of Individual Bacterial Cells through the Intermolecular Interactions with Peptide-Functionalized Solid-State Pores

Bioinspired pore sensing for selective detection of flagellated bacteria was investigated. The Au micropore wall surface was modified with a synthetic peptide designed from toll-like receptor 5 (TLR5) to mimic the pathogen-recognition capability. We found that intermolecular interactions between the TLR5-derived recognition peptides and flagella induce ligand-specific perturbations in the translocation dynamics of Escherichia coli, which facilitated the discrimination between the wild-type and flagellin-deletion mutant (ΔfliC) by the resistive pulse patterns thereby demonstrating the sensing of bacteria at a single-cell level. These results provide a novel concept of utilizing weak intermolecular interactions as a recognition probes for single-cell microbial identification.

Autoren:   Makusu Tsutsui; Masayoshi Tanaka; Takahiro Marui; Kazumichi Yokota; Takeshi Yoshida; Akihide Arima; Wataru Tonomura; Masateru Taniguchi; Takashi Washio; Mina Okochi; Tomoji Kawai
Journal:   Analytical Chemistry
Jahrgang:   2018
DOI:   10.1021/acs.analchem.7b04950
Erscheinungsdatum:   19.01.2018
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