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Materials, Vol. 11, Pages 927: A Single-Step Surface Modification of Electrospun Silica Nanofibers Using a Silica Binding Protein Fused with an RGD Motif for Enhanced PC12 Cell Growth and Differentiation

Materials, Vol. 11, Pages 927: A Single-Step Surface Modification of Electrospun Silica Nanofibers Using a Silica Binding Protein Fused with an RGD Motif for Enhanced PC12 Cell Growth and Differentiation

Materials doi: 10.3390/ma11060927

Authors: Wen Shuo Chen Ling Yu Guo Amien Mohamed Masroujeh Anna Morgan Augustine Cheng Kang Tsai Ting Yu Chin Yui Whei Chen-Yang Mong-Lin Yang

In this study, a previously known high-affinity silica binding protein (SB) was genetically engineered to fuse with an integrin-binding peptide (RGD) to create a recombinant protein (SB-RGD). SB-RGD was successfully expressed in Escherichia coli and purified using silica beads through a simple and fast centrifugation method. A further functionality assay showed that SB-RGD bound to the silica surface with an extremely high affinity that required 2 M MgCl2 for elution. Through a single-step incubation, the purified SB-RGD proteins were noncovalently coated onto an electrospun silica nanofiber (SNF) substrate to fabricate the SNF-SB-RGD substrate. SNF-SB-RGD was characterized by a combination of scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and immunostaining fluorescence microscopy. As PC12 cells were seeded onto the SNF-SB-RGD surface, significantly higher cell viability and longer neurite extensions were observed when compared to those on the control surfaces. These results indicated that SB-RGD could serve as a noncovalent coating biologic to support and promote neuron growth and differentiation on silica-based substrates for neuronal tissue engineering. It also provides proof of concept for the possibility to genetically engineer protein-based signaling molecules to noncovalently modify silica-based substrates as bioinspired material.

Autoren:   Chen, Wen Shuo; Guo, Ling Yu; Masroujeh, Amien Mohamed; Augustine, Anna Morgan; Tsai, Cheng Kang; Chin, Ting Yu; Chen-Yang, Yui Whei; Yang, Mong-Lin
Journal:   Materials
Band:   11
Ausgabe:   6
Jahrgang:   2018
Seiten:   927
DOI:   10.3390/ma11060927
Erscheinungsdatum:   30.05.2018
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