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Transpeptidation-Directed Intramolecular Bipartite Tetracysteine Display for Sortase Activity Assay

We developed a label-free method to measure Sortase activity by using transpeptidation-directed intramolecular bipartite tetracysteine display. This assay is homogenous with a detection limit of 10.7 ng/mL, and it can be applied to characterize the Sortase A (SrtA)-targeted inhibitor, providing a new approac

Autoren:   Yong Yang; Ai-jun Xu; Chun-yang Zhang
Journal:   Chemical Communication
DOI:   10.1039/C8CC04495C
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