my.chemie.de
Mit einem my.chemie.de-Account haben Sie immer alles im Überblick - und können sich Ihre eigene Website und Ihren individuellen Newsletter konfigurieren.
Captan and folpet are fungicides largely used in agriculture. They have similar chemical structures, except that folpet has an aromatic ring unlike captan. Their half-lives in blood are very short, given that they are readily broken down to tetrahydrophthalimide (THPI) and phthalimide (PI), respectively. Few authors measured these biomarkers in plasma or urine, and analysis was conducted either by gas chromatography coupled to mass spectrometry or liquid chromatography with UV detection. The objective of this study was thus to develop simple, sensitive and specific liquid chromatography–atmospheric pressure chemical ionization-tandem mass spectrometry (LC/APCI-MS/MS) methods to quantify both THPI and PI in human plasma and urine. Briefly, deuterated THPI was added as an internal standard and purification was performed by solid-phase extraction followed by LC/APCI-MS/MS analysis in negative ion mode for both compounds. Validation of the methods was conducted using spiked blank plasma and urine samples at concentrations ranging from 1 to 250 μg/L and 1 to 50 μg/L, respectively, along with samples of volunteers and workers exposed to captan or folpet. The methods showed a good linearity (R 2 > 0.99), recovery (on average 90% for THPI and 75% for PI), intra- and inter-day precision (RSD, <15%) and accuracy (<20%), and stability. The limit of detection was 0.58 μg/L in urine and 1.47 μg/L in plasma for THPI and 1.14 and 2.17 μg/L, respectively, for PI. The described methods proved to be accurate and suitable to determine the toxicokinetics of both metabolites in human plasma and urine. Figure Representative chromatograms of THPI, PI, and THPI-d in the urine of a volunteer exposed orally to captan (a) or folpet (b)
| Autoren: | Aurélie Berthet, Michèle Bouchard, Patrick Schüpfer, David Vernez, Brigitta Danuser, Cong Khanh Huynh | |
| Journal: | Analytical and Bioanalytical Chemistry | |
| Jahrgang: | 2011 | |
| DOI: | 10.1007/s00216-010-4601-z | |
| Erscheinungsdatum: | 13.01.2011 |
Nematicidal activity of essential oils: a review
Plant parasitic nematodes are the most destructive group of plant pathogens worldwide and their control is extremely challenging. Plant Essential oils (EOs) and their constituents have a great potential in nematode control since they can be developed for use as nematicides themselves or can ... mehr
For over a century, ulcer has been a major cause of morbidity and mortality. Its treatment has progressed from vagotomy to proton pump inhibitors. However, the drugs used produce many adverse effects and are less effective than they ought to be. Therefore, there is a growing interest in alt ... mehr
Endophytic fungi from medicinal plants: a treasure hunt for bioactive metabolites
Endophytic fungi are ubiquitous organisms found in the plants, residing intercellular or intracellular, at least for a portion of their lives without causing apparent symptoms of infection. Almost all plants are known to harbor endophytes. The choice of the plant to be used for exploring en ... mehr
Erstes maschinengeneriertes Chemie-Buch publiziert
Das erste maschinengenerierte Buch, das bei Springer Nature erscheint, entstand mithilfe eines Algorithmus, der von Wissenschaftlern der Goethe-Universität entwickelt wurde. Obwohl schon lange zu diesem Thema geforscht wird, betreten der Verlag und die Informatiker damit Neuland, denn es is ... mehr
Neuer Fernstudiengang Bachelor Chemie
Chemielaboranten sowie chemisch- und pharmazeutisch-technische Assistenten (CTA | PTA) sind für die praktische Arbeit im Labor gut ausgebildet, können aber häufig nur mit einem akademischen Abschluss höhere Positionen im Unternehmen einnehmen. Um diesen Berufsgruppen bessere Aufstiegschance ... mehr
LCKW im Grundwasser: Gefahr aus der Vergangenheit
Jeder Deutsche verbraucht täglich rund 120 Liter Trinkwasser. 65 Prozent davon werden aus dem Grundwasser gewonnen – einer unserer wichtigsten Ressourcen. In urbanen Räumen ist dessen Reinheit aber unter anderem durch den jahrzehntelangen Einsatz leichtflüchtiger chlorierter Kohlenwassersto ... mehr
© 1997-2021 LUMITOS AG, All rights reserved
Fachpublikationen
