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Nonlinear protein – nucleic acid crosslinking induced by femtosecond UV laser pulses in living cells


We investigate the interactions between proteins and DNA in solutions of living cells, triggered by UV, 200‐fs laser pulses. DNA‐protein crosslinking is obtained with two different techniques: conventional chemical or laser‐induced. Our results evidence a nonlinear response of the cells to the laser irradiation in the investigated intensity range. The laser induced crosslink efficiency can reach a value approximately twice higher than that obtained with ordinary chemical methods. The experimental results are very well reproduced by a simple phenomenological model based on the interplay between two‐photon absorption by DNA bases and cell damage induced by high intensity laser pulse. (© 2011 by Astro Ltd., Published exclusively by WILEY‐VCH Verlag GmbH & Co. KGaA)

Autoren:   Altucci, C.; Nebbioso, A.; Benedetti, R.; Esposito, R.; Carafa, V.; Conte, M.; Micciarelli, M.; Altucci, L.; Velotta, R.
Journal:   Laser Physics Letters
Jahrgang:   2012
Seiten:   n/a
DOI:   10.1002/lapl.201110122
Erscheinungsdatum:   16.01.2012
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