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Disposable screen printed sensor for the electrochemical detection of delta-9-tetrahydrocannabinol in undiluted saliva

Background: Cannabis has an adverse effect on the ability to drive safely, therefore a rapid disposable test for Δ 9 -tetrahydrocannabinol (Δ 9 -THC), the psychoactive component of cannabis, is highly desirable for roadside testing. Results: A screen printed carbon electrode is used for the N-(4-amino-3-methoxyphenyl)-methanesulfonamide mediated detection of Δ 9 -THC in saliva. Mediator placed in an overlayer was galvanostatically oxidized and reacted with Δ 9 -THC to give an electrochemically active adduct which could be detected by chronoamperometric reduction. Detection of 25-50 ng/mL Δ 9 -THC spiked into undiluted saliva was achieved with a response time of 30 s. A trial of the sensors with four cannabis smokers showed sensitivity of 28 %, specificity of 99 % and accuracy of 52 %. Conclusions: Rapid electrochemical detection of Δ 9 -THC in undiluted saliva has been demonstrated using a disposable sensor, however the sensitivity is lower than acceptable. Further optimization of the assay and sensor format is required to improve the sensitivity of response to Δ 9 -THC.

Autoren:   Wanklyn, Ceri; Burton, Dan; Enston, Emma; Bartlett, Carrie-Ann; Taylor, Sarah; Raniczkowska, Aleksandra; Black, Murdo; Murphy, Lindy
Journal:   Chemistry Central Journal
Band:   ${item.volume}
Ausgabe:   1
Jahrgang:   2016
Seiten:   1
DOI:   10.1186/s13065-016-0148-1
Erscheinungsdatum:   21.01.2016
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