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Lab-on-a-Drone: Toward Pinpoint Deployment of Smartphone-Enabled Nucleic Acid-Based Diagnostics for Mobile Health Care

We introduce a portable biochemical analysis platform for rapid field deployment of nucleic acid-based diagnostics using consumer-class quadcopter drones. This approach exploits the ability to isothermally perform the polymerase chain reaction (PCR) with a single heater, enabling the system to be operated using standard 5 V USB sources that power mobile devices (via battery, solar, or hand crank action). Time-resolved fluorescence detection and quantification is achieved using a smartphone camera and integrated image analysis app. Standard sample preparation is enabled by leveraging the drone’s motors as centrifuges via 3D printed snap-on attachments. These advancements make it possible to build a complete DNA/RNA analysis system at a cost of ∼$50 ($US). Our instrument is rugged and versatile, enabling pinpoint deployment of sophisticated diagnostics to distributed field sites. This capability is demonstrated by successful in-flight replication of Staphylococcus aureus and λ-phage DNA targets in under 20 ...

Autoren:   Aashish Priye; Season Wong; Yuanpeng Bi; Miguel Carpio; Jamison Chang; Mauricio Coen; Danielle Cope; Jacob Harris; James Johnson; Alexandra Keller; Richard Lim; Stanley Lu; Alex Millard; Adriano Pangelinan; Neal Patel; Luke Smith; Kamfai Chan; Victor M. Ugaz
Journal:   Analytical Chemistry
Jahrgang:   2016
DOI:   10.1021/acs.analchem.5b04153
Erscheinungsdatum:   21.04.2016
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