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Development of Hollow-Fiber Liquid-Phase Microextraction Method for Determination of Urinary trans,trans-Muconic Acid as a Biomarker of Benzene Exposure

For the first time, hollow-fiber liquid-phase microextraction combined with high-performance liquid chromatography–ultraviolet was used to extract trans,trans-muconic acid, in urine samples of workers who had been exposed to benzene. The parameters affecting the metabolite extraction were optimized as follows: the volume of sample solution was 11 mL with pH 2, liquid membrane containing dihexyl ether as the supporter, 15% (w/v) of trioctylphosphine oxide as the carrier, the time of extraction was 120 minutes, and stirring rate was 500 rpm. Organic phase impregnated in the pores of a hollow fiber was extracted into 24 µL solution of 0.05 mol L⁻¹ Na₂CO₃ located inside the lumen of the fiber. Under optimized conditions, a high enrichment factor of 153–182 folds, relative recovery of 83%–92%, and detection limit of 0.001 µg mL⁻¹ were obtained. The method was successfully applied to the analysis of ttMA in real urine samples.

Autoren:   Farhad Ghamari, Abdulrahman Bahrami, Yadollah Yamini, Farshid Ghorbani Shahna and Abbas Moghimbeigi
Journal:   Analytical Chemistry Insights
Band:   2016
Ausgabe:   1
Jahrgang:   2016
Seiten:   65
DOI:   10.4137/ACI.S40177
Erscheinungsdatum:   13.09.2016
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