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Author Correction: High-speed volumetric imaging of neuronal activity in freely moving rodents

21.05.2018 | Oliver Skocek; Tobias Nöbauer; Lukas Weilguny; Francisca Martínez Traub; Chuying Naomi Xia; Maxim I. Molod ..., Nature Methods, 2018

In the version of this Brief Communication originally published online, ref. 21 included details for a conference paper (Pegard, N. C. et al. Paper presented at Novel Techniques in Microscopy: Optics in the Life Sciences, Vancouver, BC, Canada, 12–15 April 2015). The correct reference is the ...


Hyaluronan negatively regulates vascular calcification involving BMP2 signaling

21.05.2018 | Yonglun Kong; Qingchun Liang; Yanting Chen; Pingzhen Yang; Xiaoyu Liu; Yining Li; Siyuan Feng; Ji Wu; Wantao Liu; Ji ..., Laboratory Investigation, 2018

Vascular calcification is a highly regulated biological process similar to bone formation involving osteogenic differentiation of vascular smooth muscle cells (VSMCs). Hyaluronan (HA), a major structural component of the extracellular matrix in cartilage, has been shown to inhibit osteoblast ...


Author Correction: Ultrahard carbon film from epitaxial two-layer graphene

21.05.2018 | Yang Gao; Tengfei Cao; Filippo Cellini; Claire Berger; Walter A. Heer; Erio Tosatti; Elisa Riedo; Angelo Bongiorno, Nature Nanotechnology, 2018

Author Correction: Ultrahard carbon film from epitaxial two-layer graphene Author Correction: Ultrahard carbon film from epitaxial two-layer graphene, Published online: 21 May 2018; doi:10.1038/s41565-018-0158-3 Author Correction: Ultrahard carbon film from epitaxial two-layer graphene


Broadband, electrically tunable third-harmonic generation in graphene

21.05.2018 | Giancarlo Soavi; Gang Wang; Habib Rostami; David G. Purdie; Domenico De Fazio; Teng Ma; Birong Luo; Junjia Wang; Ann ..., Nature Nanotechnology, 2018

Optical harmonic generation occurs when high intensity light (>1010 W m–2) interacts with a nonlinear material. Electrical control of the nonlinear optical response enables applications such as gate-tunable switches and frequency converters. Graphene displays exceptionally strong light–matter ...


Single entity resolution valving of nanoscopic species in liquids

21.05.2018 | Patric Eberle; Christian Höller; Philipp Müller; Maarit Suomalainen; Urs F. Greber; Hadi Eghlidi; Dimos Po ..., Nature Nanotechnology, 2018

Investigating biological and synthetic nanoscopic species in liquids, at the ultimate resolution of single entity, is important in diverse fields1–5. Progress has been made6–10, but significant barriers need to be overcome such as the need for intense fields, the lack of versatility in operating ...


Mapping the physical network of cellular interactions

21.05.2018 | Jean-Charles Boisset; Judith Vivié; Dominic Grün; Mauro J. Muraro; Anna Lyubimova; Alexander Oudenaarden, Nature Methods, 2018

A cell’s function is influenced by the environment, or niche, in which it resides. Studies of niches usually require assumptions about the cell types present, which impedes the discovery of new cell types or interactions. Here we describe ProximID, an approach for building a cellular network ...


CRISPR off-target analysis in genetically engineered rats and mice

21.05.2018 | Keith R. Anderson; Maximilian Haeussler; Colin Watanabe; Vasantharajan Janakiraman; Jessica Lund; Zora Modrusan; Jer ..., Nature Methods, 2018

Despite widespread use of CRISPR, comprehensive data on the frequency and impact of Cas9-mediated off-targets in modified rodents are limited. Here we present deep-sequencing data from 81 genome-editing projects on mouse and rat genomes at 1,423 predicted off-target sites, 32 of which were ...


High-speed photothermal off-resonance atomic force microscopy reveals assembly routes of centriolar scaffold protein SAS-6

21.05.2018 | Adrian P. Nievergelt; Niccolò Banterle; Santiago H. Andany; Pierre Gönczy; Georg E. Fantner, Nature Nanotechnology, 2018

The self-assembly of protein complexes is at the core of many fundamental biological processes 1 , ranging from the polymerization of cytoskeletal elements, such as microtubules 2 , to viral capsid formation and organelle ...


Involvement of inhibitor kappa B kinase 2 (IKK2) in the regulation of vascular tone

21.05.2018 | Youngin Kwon; Soo-Kyoung Choi; Seonhee Byeon; Young-Ho Lee, Laboratory Investigation, 2018

Inhibitor kappa B kinase 2 (IKK2) plays an essential role in the activation of nuclear factor kappa B (NF-kB). Recently, it has been suggested that IKK2 acts as a myosin light chain kinase (MLCK) and contributes to vasoconstriction in mouse aorta. However, the underlying mechanisms are still ...


The tumor suppressor DLC1 inhibits cancer progression and oncogenic autophagy in hepatocellular carcinoma

21.05.2018 | Hui-Ta Wu; Cheng-Rong Xie; Jie Lv; He-Qiang Qi; Fei Wang; Sheng Zhang; Qin-Liang Fang; Fu-Qiang Wang; Yu-Yan Lu; Zhe ..., Laboratory Investigation, 2018

Downregulation of deleted in liver cancer 1 (DLC1) is associated with poor prognosis of various cancers, but its functional mechanisms in hepatocellular carcinoma (HCC) remains unclear. In the present study, we investigated the roles of DLC1 in tumor progression and autophagy of HCC. We found ...


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