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131 Aktuelle Fachpublikationen in Analytical Chemistry Insights

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Insight into Biochemical Characterization of Plant Sesquiterpene Synthases

04.10.2016 | Tom Manczak and Henrik Toft Simonsen, Analytical Chemistry Insights, 2016

A fast and reproducible protocol was established for enzymatic characterization of plant sesquiterpene synthases that can incorporate radioactivity in their products. The method utilizes the 96-well format in conjunction with cluster tubes and enables processing of >200 samples a day. Along with ...

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Stability-Indicating High-Performance Liquid Chromatography Assay for the Determination of Sulthiame in Pharmaceutical Dosage Forms

16.09.2016 | Ammar Haidar, Sofiane Kabiche, Elyes Majoul, Issa-Bella Balde, Jean-Eudes Fontan, Salvatore Cisternino and Joël ..., Analytical Chemistry Insights, 2016

A stability-indicating assay by reversed-phase high performance liquid chromatography method was developed and validated for the determination of sulthiame (STM). The chromatographic separation was achieved on a reversed-phase NovaPack C18 column and an isocratic mobile phase consisting of ...

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Development of Hollow-Fiber Liquid-Phase Microextraction Method for Determination of Urinary trans,trans-Muconic Acid as a Biomarker of Benzene Exposure

13.09.2016 | Farhad Ghamari, Abdulrahman Bahrami, Yadollah Yamini, Farshid Ghorbani Shahna and Abbas Moghimbeigi, Analytical Chemistry Insights, 2016

For the first time, hollow-fiber liquid-phase microextraction combined with high-performance liquid chromatography–ultraviolet was used to extract trans,trans-muconic acid, in urine samples of workers who had been exposed to benzene. The parameters affecting the metabolite extraction were ...

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Extraction of Honey Polyphenols: Method Development and Evidence of Cis Isomerization

20.08.2016 | Thibaut Istasse, Nicolas Jacquet, Thomas Berchem, Eric Haubruge, Bach Kim Nguyen and Aurore Richel, Analytical Chemistry Insights, 2016

Honey polyphenols have been studied with the objective of relating honeys to their floral sources. Initially synthesized by plant, these polyphenols can be found in the plant’s nectar, which are collected by bees, which convert the nectar into honey. Consequently, polyphenols constitute minor ...

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On the Conflicting Estimations of Pigment Site Energies in Photosynthetic Complexes: A Case Study of the CP47 Complex

07.06.2016 | Tonu Reinot, Jinhai Chen, Adam Kell, Mahboobe Jassas, Kevin C. Robben, Valter Zazubovich and Ryszard Jankowiak, Analytical Chemistry Insights, 2016

We focus on problems with elucidation of site energies (E₀ⁿ)for photosynthetic complexes (PSCs) in order to raise some genuine concern regarding the conflicting estimations propagating in the literature. As an example, we provide a stern assessment of the site energies extracted from fits to ...

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Removal of 4-Nitrophenol from Water Using Ag-N-P-Tridoped TiO2 by Photocatalytic Oxidation Technique

13.04.2016 | Temesgen Achamo and O.P. Yadav, Analytical Chemistry Insights, 2016

Photocatalytic oxidation using semiconductor nanoparticles is an efficient, eco-friendly, and cost-effective process for the removal of organic pollutants, such as dyes, pesticides, phenols, and their derivatives in water. In the present study, nanosize Ag–N–P-tridoped titanium(IV) oxide (TiO₂) ...

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Quality Issues of Research Antibodies

29.03.2016 | Michael G. Weller, Analytical Chemistry Insights, 2016

According to several recent studies, an unexpectedly high number of landmark papers seem to be not reproducible by independent laboratories. Nontherapeutic antibodies used for research, diagnostic, food analytical, environmental, and other purposes play a significant role in this matter. Although ...

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Development and Validation of Stability-Indicating RP-HPLC Method for Simultaneous Determination of Metformin HCl and Glimepiride in Fixed-Dose Combination

15.03.2016 | Pradnya N. Vaingankar and Purnima D. Amin, Analytical Chemistry Insights, 2016

A simple reversed-phase high-performance liquid chromatography method was developed and validated for simultaneous determination of Metformin hydrochloride (MET) and Glimepiride (GLM) in combination and estimation of their principal degradation products. The separation was achieved using JASCO ...

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Electrochemical Behavior and Voltammetric Determination of a Manganese(II) Complex at a Carbon Paste Electrode

27.01.2016 | Sophia Karastogianni and Stella Girousi, Analytical Chemistry Insights, 2016

Investigation of the electrochemical behavior using cyclic voltammetry and detection of [Mn²⁺(thiophenyl-2-carboxylic acid)₂ (triethanolamine)] with adsorptive stripping differential pulse voltammetry. The electrochemical behavior of a manganese(II) complex [Mn²⁺(thiophenyl-2-carboxylic ...

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Sample Preparation of Eggs From Laying Hens Using QuEChERS Dispersive Extraction for the Simultaneous Determination of Melamine and Cyromazine Residues by HPLC-DAD

22.12.2015 | Niki Tsartsali and Victoria F. Samanidou, Analytical Chemistry Insights, 2015

A quick, easy, cheap, effective, rugged, and safe (QuEChERS) dispersive extraction method is proposed herein for the isolation and cleanup of melamine and cyromazine from chicken egg yolk. Analytes are determined by high-performance liquid chromatography using photodiode array detector after ...

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