19.01.2018 | Chunfeng Cheng; Chunmei Zhang; Xiaohui Gao; Zhihua Zhuang; Cheng Du; Wei Chen, Analytical Chemistry, 2018

In this study, two-dimensional (2D) and three-dimensional (3D) freestanding reduced graphene oxide-supported Cu2O composites (Cu2O-rGO) were synthesized via simple and cost-efficient hydrothermal and filtration strategies. The structural characterizations clearly showed that highly porous 3D ...

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19.01.2018 | Sean D. Gates; Richard C. Condit; Nissin Moussatche; Benjamin J. Stewart; Alexander J. Malkin; Peter K. Weber, Analytical Chemistry, 2018

High-lateral-resolution secondary ion mass spectrometry (SIMS) has the potential to provide functional and depth resolved information from small biological structures, such as viral particles (virions) and phage, but sputter rate and sensitivity are not characterized at shallow depths relevant to ...

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19.01.2018 | Wei Ding; Difei Qiu; Mark S. Bolgar; Scott A. Miller, Analytical Chemistry, 2018

Monoclonal antibodies (mAbs) are experiencing accelerated development in the pharmaceutical industry. Utilization of middle-up LC-MS methodology can provide detailed characterization of mAbs via reduction and/or enzymatic cleavage of the mAb into smaller protein fragments. However, under typical ...

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19.01.2018 | Bertrand Plainchont; Daisy Pitoux; Mathieu Cyrille; Nicolas Giraud, Analytical Chemistry, 2018

We propose an original concept to measure accurately enantiomeric excesses on proton NMR spectra, which combines high-resolution techniques based on a spatial encoding of the sample, with the use of optically active weakly orienting solvents. We show that it is possible to simulate accurately ...

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19.01.2018 | Bin Yang; Beibei Chen; Man He; Xiao Yin; Chi Xu; Bin Hu, Analytical Chemistry, 2018

Development of multimodal detection technologies for accurate diagnosis of cancer at early stages is in great demand. In this work, we report a novel approach using an aptamer-based dual-functional probe for rapid, sensitive, and specific counting and visualization of MCF-7 cells by inductively ...

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19.01.2018 | Makusu Tsutsui; Masayoshi Tanaka; Takahiro Marui; Kazumichi Yokota; Takeshi Yoshida; Akihide Arima; Wataru Tonomura; ..., Analytical Chemistry, 2018

Bioinspired pore sensing for selective detection of flagellated bacteria was investigated. The Au micropore wall surface was modified with a synthetic peptide designed from toll-like receptor 5 (TLR5) to mimic the pathogen-recognition capability. We found that intermolecular interactions between ...

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19.01.2018 | Tetsuo Sasaki; Tomoaki Sakamoto; Makoto Otsuka, Analytical Chemistry, 2018

Quantitative detection of impurities in organic crystals was demonstrated by accurately measuring absorption frequencies using a continuous wave gallium phosphide terahertz spectrometer. THz spectra of l-asparagine monohydrate doped with l-aspartic acid at 0.05 to 12.5 wt % were obtained at 10 K. ...

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19.01.2018 | Nick DeGraan-Weber; James Patrick Reilly, Analytical Chemistry, 2018

Aminoethylation of cysteines can provide enzymatically cleavable sites. The ability to obtain peptides containing antibody complementarity determining regions (CDRs) with aminoethylated cysteines was investigated. Because cysteines are often located N-terminal to CDRs, digestion with Lys-N ...

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18.01.2018 | Erica Brunelle; Crystal Huynh; Eden Alin; Morgan Eldridge; Anh Minh Le; Lenka Halámková; Jan Halámek, Analytical Chemistry, 2018

Analytical Chemistry DOI: 10.1021/acs.analchem.8b00039

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18.01.2018 | Bing Bo; Tian Zhang; Yiting Jiang; Haiyan Cui; Peng Miao, Analytical Chemistry, 2018

Abnormal expression of miRNAs always occurs in solid tumors. Thus, it is critical to sensitively and selectively detect such biomarkers for the diagnosis and prognosis of diseases. Here, we report a biosensing scheme for the determination of miRNA with triple signal amplification based on ...

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